In vitro supplementation of lycopene to bovine spermatozoa: effects on motility, viability and superoxide production
Journal Title: Animal Science Papers and Reports - Year 2016, Vol 34, Issue 4
Abstract
This study was designed to assess the effects of lycopene (LYC) on motility, viability and superoxide production of bovine spermatozoa during short-term (0h, 2h, 6h) and long-term (12h, 24h) in vitro culture periods. Semen samples were collected from 20 adult breeding bulls and diluted in saline supplemented with 2000, 1000, 500, 200, 100, 50, 10, 5 and 1 μM/L LYC. Spermatozoal motion parameters were examined using the SpermVisionTM Computer Assisted Semen Analyzer (CASA) system. Cell viability was measured using the metabolic activity MTT assay, while the nitroblue-tetrazolium (NBT) test was used to assess intracellular superoxide formation. The CASA analysis revealed that 100 - 2000 μM/L LYC were able to prevent a rapid decline of spermatozoal motion (P<0.001 with respect to 200-2000 μM/L LYC; P<0.01 given 100 μM/L LYC). At the same time, supplementation of 1000 and 2000 μM/L LYC led to a significant preservation of cell viability (P<0.05 in the case of Time 0h and 2h; P<0.01 at Time 6h and 12h; P<0.001 with respect to Time 24h). All applied LYC concentrations significantly reduced the intracellular superoxide production, particularly at Times 12h (P<0.01 in the case of 1000 and 2000 μM/L LYC; P<0.05 with respect to 1-500 μM/L LYC) and 24h (P<0.001 in the case of 1000 and 2000 μM/L LYC; P<0.01 with respect to 500 μM/L LYC; P<0.05 given 1-200 μM/L LYC). The results indicate that lycopene may enhance spermatozoal activity and protect against cellular deterioration resulting from exposure to the in vitro environment.
Authors and Affiliations
Eva Tvrda, Norbert Lukac, Tomas Jambor, Jana Lukacova, Faridullah Hashim, Peter Massanyi
Keywords
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