In Vivo and In Vitro Cytotoxicity and Mutagenicity Considerations of Poly (Amido Amine) Dendrimer

Journal Title: Journal of Reports in Pharmaceutical Sciences - Year 2015, Vol 4, Issue 1

Abstract

A lot of chemicals such as poly (amidoamine) (PAMAM-NH2) dendrimers have pharmaceutical applications, but the major problem with PAMAM-NH2 is their cytotoxicity and mutagenicity. In this research, we have investigated the cytotoxicity and mutagenicity of various generations of PAMAM-NH2 (G2.0, G3.0, G4.0, and G5.0). The cytotoxicity of PAMAM-NH2 at the dilutions of 0.01, 0.001 and 0.0001(W/W) to human mesenchymal stem cells (MSCs) and human gastric adenocarcinoma (AGS) cells was determined using the standard methyl-thiazol-tetrazoliumA (MTT)A assay. To determine mean lethal dose (LD50) of PAMAM-NH2 at doses of 30, 47, 73.5, 115 and 180 mg/kg, 125 Bagg albino/cA (BALB/c) mice (8–10 weeks of age, weighing approximately 20 g) were used and also, for determining the mutagenicity effect of PAMAM-NH2, 50I¼L volume of this substance in the Ame’s test with S. typhimurium was applied.A In the MTT assay the most toxic effects, on both of the cell lines, were related to the time when G2.0, G3.0, G4.0 and G5.0 were applied at different dilution of 0.01, 0.001, 0.0001(W/W), respectively.A LD50 was determined 73.5 mg/kg. Also in the Ame’s test, the number of reverted colonies was increased by applying higher generations and inhibition percentages of PAMAM-NH2 that were 69.47%, 68.42%, 64.210% and 64.21% for G2.0, G3.0, G4.0 and G5.0, respectively. According to these results, PAMAM-NH2 generations had cytotoxicity effects on MSCs and AGS cells; also toxicity and mutagenicity of the substance were proved in mice and S. typhimurium, respectively. So in order to use PAMAM-NH2 in pharmaceuticals, it must be subjected to various tests to ensure its safety.

Authors and Affiliations

Keywords

Related Articles

Development and validation of a new method for determination of topiramate in bulk and pharmaceutical formulation using high performance liquid chromatography-UV detection after pre-column derivatization

In published high performance liquid chromatographic (HPLC) methods for analysis of topiramate (TPM) in pharmaceutical dosage forms and raw materials Refractive Index Detector (RID) has been used which is not available i...

Chemical Composition of Volatile Oils from the Endocarp and Hulls of Persian Bay Laurel Fruit: A Fragrant Herb Used In Traditional Iranian Medicine

The hydro-distilled volatile oils, obtained from dried endocarp and hulls of Persian bay laurel (Laurus nobilis var. angustifolia) from Laueaceae family were analyzed by GC/MS. Thirteen and twelve compounds consisting 81...

Effects of Migriheal® on Plasma Proteome of Patients with Migraine Headaches

Recently, based on herbal medicine of Iran, an herbal drug with medical effects named MigriHeal®, has been registered; it helps in prevention of migraine headache strokes. In this study, MigriHeal was administered for pu...

Chemical composition and general toxicity of essential oil extracted from the stalks and flowers of Rheum ribes L. growing in Iran

The composition of the essential oil from the stalks and flowers of Rheum ribes L., growing in Iran, were extracted by hydrodistillation and analyzed by GC-MS. Thirty constituents representing 93.84% of Rheum oil were id...

Detection of corticosteroid compounds and phosphodiester- ase inhibitors (PDH-5) as counterfeit in herbal products available in Iranian market by HPLC method

Weight-gain and potency enhancing drugs are the most popular herbal products in Iran which are easily available via the internet and through illegal markets. The content of some of these popularly purchased products were...

Download PDF file
  • EP ID EP340228
  • DOI -
  • Views 51
  • Downloads 0

How To Cite

(2015). In Vivo and In Vitro Cytotoxicity and Mutagenicity Considerations of Poly (Amido Amine) Dendrimer. Journal of Reports in Pharmaceutical Sciences, 4(1), -. https://europub.co.uk./articles/-A-340228