Induction and callus formation of Anthurium andraeanum variety ´Lambada ’ from foliar sections of in vitro plants
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 1
Abstract
Foliar sections of in vitro plants of Anthurium andraeanum variety ‘Lambada’ were used as explants for the induction and formation of callus. The formation of callus was evaluated in two culture mediums (MS and Nitsch, modified) to which the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) were added in four concentrations 2.26, 4.52, 6.78 and 9.04 μM and the addition of 6-Benzilaminopurine (6-BAP) to a concentration of 2.22 μM keeping it constant for both culture mediums. The treatments were placed in darkness at 28±2oC during 45 days. Characteristics of the callus, such as colour and form, were also described . The results showed, in the statistical analysis, a significant interaction among the factors in study (concentrations of the growth regulator and type of culture medium). The calluses obtained presented translucent cream yellow colour with nodular structures. Callus were formed in the two culture medium used, however, the biggest explants percentage that allowed the formation of these structures was observed in the culture medium MS modified when a concentration of 6.78 μM 2,4-D was used . It was demonstrated that it is possible to use foliar sections of in vitro plants to form callus in Anthurium andraeanum variety ‘Lambada’ in the culture medium MS with the nitrates reduced in a half and 2,4-D.
Authors and Affiliations
Nydia del Rivero , Daniel Agramonte , Raúl Barbón , Wilder Camacho, Raúl Collado , Felipe Jiménez-Terry , Marta Pérez, Odalys Gutiérrez
Keywords
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