Influence of aminoacids on Bacillus genus bacteria spore germination
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 33, Issue
Abstract
The study applies experimental approaches to spore germination to determine whether the germinants will affect the two different species B. cereus and B. anthracis using in vitro tests. Carrying out studies on the vaccine strain of the B. anthracis, we used different combinations of amino acids to study possible ways to improve spore germination. The best results were obtained using inosine and L-serine as well as inosine with L-alanine and L-valine (0.1 mM aqueous solution of an equal ratio of amino acids was used). Analysis of the results of the studies showed that part of spores germinated in nutrient media after treatment with a mixture of amino acids reached 86.2% compared with 58% in the control. The variability of these values for tested strains ranged from ± 3.8% to ± 13.6%. We also found that the percentage of germinated spores does not increase significantly in time after the first 48 hours from the start of cultivation
Authors and Affiliations
S. Krylenko, Oleksandr Tarasov, Natalia Hudz, Maria Babkina, Svitlana Tereschenko
Keywords
Related Articles
- EP ID EP411596
- DOI 10.31073/vet_biotech33-18
- Views 133
- Downloads 0
Development and validation of real time polymerase chain reaction for detection DNA of pathogenic Leptospira
Introduction. Currently the polymerase chain reaction (PCR) development and use are conducted for the detection of the genetical and pathological characteristics of Leptospira and leptospirosis diagnostics. Due to this m...
Development of the test kit for the differential diagnostics of african and classical swine fever by RT-PCR in real time
Introduction. African swine fever (ASF) and Classical swine fever (CSF) are two transboundary contagious viral swine diseases with high mortality rates and huge economic impacts. ASF cannot be differentiated from CSF by...
Determination of the remaining amounts of baquiloprim and trimethoprim residue in meat, liver and kidney by microbiological method
Introduction. One of the priority areas of the state policy concerning public health nutritio is provision of foodstuff safety. Thus, features of their composition are taken into account, since, in addition to plastic ma...
Helminthofauna of wild ungulates: ecology, species composition, distribution (review)
Introduction. Conservation of biodiversity in natural and transformed ecosystems under anthropogenic influence is one of the priorities of the modern population and applied ecology. Of particular urgency is the issue in...
Peculiarities of pathogenesis in case of simuliidotoxicosis of cattle
Introduction. The black-flies of the Simuliidae family are particularly dangerous among the bloodsucking dipterans insects. They cause pathological phenomena in different kinds of warm-blooded animals, recognized as simu...