Influence of Different Buffers on Embryo Growth in In-vitro fertilization
Journal Title: Chettinad Health City Medical Journal - Year 2017, Vol 6, Issue 4
Abstract
Introduction: The human reproduction is achieved as the domino effect of successful synchronization and commu- nication between the embryo and the uterine endometrium. Here the embryo is thought to be the most important factor for achieving a pregnancy in assisted reproductive technology (ART). These embryos were cultured in culture media containing various vital components, of which the buffers for maintaining the pH play an imperative role. The bicarbonate buffer being the oldest & most commonly used buffer in Assisted Reproductive Technology (ART) for handling gametes and culturing them as embryos is now replaced by 4-2-hydroxyethyl-1-piperazine ethane sulfonic acid (HEPES) & 3-N-morpholino propane sulfonic acid (MOPS) organic based buffers for collec- tion and manipulation of gametes. Aim: This study was aimed to observe the growth potential of embryos in culture media containing organic buffers such as HEPES & MOPS. Materials and Methods: The male and female Swiss albino mice which imprints similar genetic pattern as humans were used for the study. The female mice were stimulated for super-ovulation, sacrificed, Cumulus Oocyte Complex (COC) were retrieved from ampulla. The male mice of appropriate age were selected sacrificed and spermatozoa were retrieved from the testis. These retrieved cumulus complex were inseminated with spermato- zoa at standard concentration ( In-Vitro Fertilization) and culture of embryos were done as groups I, II, III in culture media containing bicarbonate (CO2), HEPES & MOPS respectively. Study design: Prospective study Study duration: October 2016 to May 2017; for a period of 8 months Study setting: Dept. of Reproductive Medicine & Andrology, Chettinad Super Speciality Hospital, Chennai. Result: The fertilization rate in bicarbonate, HEPES, MOPS buffer based culture media were 60%, 51.42% & 0% respectively. The oocytes inseminated in MOPS based culture media failed to survive. Conclusion: This study shows that HEPES can be used as substitute to bicarbonate but cannot be replaced.
Authors and Affiliations
Rajalakshmi C, Asha B, Ranjani S, Ramesh Raja
Keywords
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