Influence of General Cooling on Morphometric Indicators of Ovarians and Brown Fatty Tissue in Rats in Experiment
Journal Title: Український журнал медицини, біології та спорту - Year 2017, Vol 6, Issue 4
Abstract
To study the effect of total cooling on the morphometric parameters of the ovaries and brown fatty tissue (BFT), we carried out studies of 40 white 4-week-old female rats of the Vistar line, weighing 30-40, which were divided into 5 groups: 1st-8 rats, which were subjected to constant cold exposure (CCE); the 2 nd – 8 rats who were injected with dehydroepiandrostenediol-sulfate (DHA-sulfate) dissolved in 0.2 ml of purified and sterilized olive oil against the background of CCE; the 3rd – 8 rats, who were induced with experimental polycystic ovary syndrome (PCOS) by administration of only DHA-sulfate, without CCE; the 4 th group – 8 animals, who received only olive oil without DHA-sulfate and CCE; and the 5th group comprised 8 rats, who did not receive anything: neither intact control nor CCE exposure. The CCE was carried out by holding the animals for 4 hours in a chamber where the light regime and temperature of + 4 ° C. The remaining 20 h animals were in normal conditions of ambient temperature and light conditions. The rats were cooled daily for 25 days. At the 26th day, the animals were sacrificed; the ovaries and BFT were taken from the interscapular area. The organs were fixed in 4% paraformaldehyde for 4 hours, after which it was transferred for 12 hours to a 25% sucrose solution on phosphate buffered saline (PBS). The organs in the mounting medium Tissue-Tek, were frozen and before the preparation of the cryostat sections were stored in nitrogen liquid. Histological examination was performed on BFT and ovaries of experimental animals. For the preparation of cryostat sections, the organs were removed from the low-temperature storage and fabric sections were made with the thickness of 5 μm on the cryovicrotome MEV (Germany). Sections were stained with hematoxylin and eosin according to the standard procedure. The number of adipocytes of BFT of different types was counted. The daily placement of rats in conditions of general cooling (4 h at + 4 ° C) led to a change in the cytomorphological characteristics of BFT. In group 1, there was a decrease in the number of type 1 and type 2 adipocytes in comparison with intact tissue. Visually, the tissue acquired a denser package and a pronounced brownish-brown color. Animals of the 2nd group, who underwent DHA- sulfate administration in conditions of general cooling, had sign characteristics of intact tissue: cells with large fat vacuoles were preserved. In this case, areas with tightly packed adipocytes with small fatty inclusions (A1) were alternated with zones represented by type 1 and type 2 adipocytes. Against the background of the introduction of DHA-sulfate without CCE (group 3), the main morphological features characteristic of groups 4 and 5 were preserved in BFT. Morphometric analysis allowed making a quantitative assessment of the changes in the cytomorphological profile of the BFT under the applied effects. A significant decrease in the number of A1 type cells in BFT animals with CCE (group 1) and A3 cells in BFT animals with CCE + DHA-sulfate (group 2) was found in comparison with the intact control. Changes occurred not only in the quantitative ratio of the types of adipocytes, but also in terms of area and diameter of lipid droplets. Summarizing the results, it can be noted that under the influence of cold occur adaptive reactions in the BFT animals. These reactions are aimed at mobilizing lipid stocks to generate heat. It was found that the administration of DHA sulfate to 4-week-old female rats for 25 days leads to the development of cysts in the ovaries. Activation of BFT in rats subjected to CCE (at +4 C for 4 hours) was performed for 25 days. CCE on the background of the introduction of DHA-sulfate prevents the development of cystic changes in the ovaries of rats.
Authors and Affiliations
I. Kuzmina, M. Zhulikova, G. Bozhok
Keywords
Related Articles
- EP ID EP273088
- DOI -
- Views 101
- Downloads 0
The Immun-Biochemical Status Changes of Critical Patients with a Hemorrhagic Disease during Intensive Therapy
In the structure of a cerebral stroke hemorrhagic stroke is the most destructive type of stroke leading to death and severe disability of patients. Mortality due to hemorrhagic stroke varies from 25% in persons who do no...
ПЕРСПЕКТИВИ ВПРОВАДЖЕННЯ СИСТЕМИ ОХОРОНИ ПСИХІЧНОГО ЗДОРОВ’Я НА ПЕРВИННОМУ РІВНІ
Стаття присвячена аналізу законодавства у сфері охорони психічного здоров’я на рівні первинної медичної допомоги. Нами з’ясовані особливості охорони психічного здоров’я на первинному рівні надання медичної допомоги в зак...
Angiolon Influence on the Morphofunctional Characteristics of Rats’ Endotheliocytes in Chronic Cardiac Insufficiency
Endotheliocytes damage may be diagnosed in chronic cardiac insufficiency besides of cardiac activity and disturbance. The aim of the study is to examine the angiolin influence on the morphofunctional characteristics of r...
Examination of Adhesive Properties of C. diphtheriae Surface Antigens Obtained by the Cascade Disintegration of Microbial Cells by Different Physical Factors
The purpose of the article is to examine new generation vaccines against diphtheria infection on the basis of the native bacterial antigenic complexes isolation by cascade disintegration with the use of physical factors....
The Reamberin’s Usage Effectiveness with the Purpose of Cognitive Functions’ Correction in the Acute Period of Polytrauma
Injury is one of the causes of critical states and cognitive impairments development as well as their consequences. Cognitive impairments that occur in critical patients can not only significantly slow down the recovery...