Influence of mixing parameters in STAR automatic sample processing instruments on the detection results of internal controls
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 3
Abstract
Objective To explore the significance of blending internal controls by automatic sample processing instruments in the enzyme linked immunosorbent assay (ELISA). Methods The internal controls were vortexed and mixed before the test, and then were added to the same ELISA plate by the STAR automatic sample processing instruments under the same detection conditions. The difference of S/CO value of internal controls with and without sufficient blending via the sampling needle and their frequency distribution were compared. Internal controls that were greatly affected by mixing parameters were submitted to the same test with different batches of reagents from the same manufacturer, and the results were analyzed for consistency. Results The S/CO value of anti-HCV internal controls without blending using adding sample needle was significantly lower than that of quality control samples with sufficient blending (P<0.000 1). The S/CO values of unmixed internal controls concerning anti-TP and anti-HIV detection given by some detection systems were also different from the values of mixed internal controls (P<0.05). Some of the S/CO values of the anti-HCV internal controls without mixing were distributed within the interval of less than 2. Different batches of reagents from the same manufacturer were used to detect anti-HCV internal controls, and there were differences in the partial detection values between the mixed and unmixed internal controls (P<0.05). Conclusion Although the internal controls were mixed by vortex shock before the test, the detection results of some internal controls will still be affected when the STAR automatic sample processing instruments does not set the mixing parameters for internal controls.
Authors and Affiliations
Jing GAO, Jianhuai JIN, Youshan XU
Keywords
Related Articles
- EP ID EP738505
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.03.006
- Views 51
- Downloads 0
Changes in hematological parameters of long-term high-frequency platelet donors
Objective To understand the effect of long-term high-frequency platelet donation on the health, safety and platelet quality of blood donors. Methods From August 2020 to July 2022, blood donors who donated platelets for s...
Expert consensus on unmanned aerial vehicle blood transportation in Shenzhen
The unmanned aerial vehicle (UAV) blood transportation service is a convenient blood delivery way provided by the Shenzhen Blood Centre. It is also an essential part of the “Air-ground Integrated” blood delivery model. U...
Skin injury around enterostomy cured by allogeneic platelet-rich plasma: a report of two cases
Objective To investigate the feasibility of allogeneic platelet-rich plasma (PRP) for the treatment of skin injury around enterostomy. Methods The treatment process by PRP of 2 patients with skin injury around enterostom...
Serological follow-up of anti-SARS-CoV-2 antibodies in blood donors after COVID-19 vaccine booster dose and breakthrough infections in Fuzhou
Objective To analyze the dynamics of specific SARS-CoV-2 IgG antibodies in blood donors in Fuzhou area after receiving booster doses of inactivated COVID-19 vaccine and breakthrough infections, and to provide evidence fo...
Research on a new microtubule technology of reagent bursting for crossmatching test
Objective To develop a quick and accurate crossmatching test technology without the power equipment and additional reagents before blood transfusion, so as to improve the timeliness and safety of blood transfusion treatm...