Influence of mixing parameters in STAR automatic sample processing instruments on the detection results of internal controls
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 3
Abstract
Objective To explore the significance of blending internal controls by automatic sample processing instruments in the enzyme linked immunosorbent assay (ELISA). Methods The internal controls were vortexed and mixed before the test, and then were added to the same ELISA plate by the STAR automatic sample processing instruments under the same detection conditions. The difference of S/CO value of internal controls with and without sufficient blending via the sampling needle and their frequency distribution were compared. Internal controls that were greatly affected by mixing parameters were submitted to the same test with different batches of reagents from the same manufacturer, and the results were analyzed for consistency. Results The S/CO value of anti-HCV internal controls without blending using adding sample needle was significantly lower than that of quality control samples with sufficient blending (P<0.000 1). The S/CO values of unmixed internal controls concerning anti-TP and anti-HIV detection given by some detection systems were also different from the values of mixed internal controls (P<0.05). Some of the S/CO values of the anti-HCV internal controls without mixing were distributed within the interval of less than 2. Different batches of reagents from the same manufacturer were used to detect anti-HCV internal controls, and there were differences in the partial detection values between the mixed and unmixed internal controls (P<0.05). Conclusion Although the internal controls were mixed by vortex shock before the test, the detection results of some internal controls will still be affected when the STAR automatic sample processing instruments does not set the mixing parameters for internal controls.
Authors and Affiliations
Jing GAO, Jianhuai JIN, Youshan XU
Keywords
Related Articles
- EP ID EP738505
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.03.006
- Views 60
- Downloads 0
Application of monocyte monolayer assay on hemolytic disease of fetus and newborn caused by IgG anti-M
Objective To elucidate the prediction ability of monocyte monolayer assay(MMA) used in hemolytic disease of fetus and newborn(HDFN) caused by IgG anti-M. Methods Plasma from eight pregnant women containing IgG anti-M wer...
Demographic difference and influencing factors of motivations between whole blood and plasmapheresis donors: a comparative study
Objective To explore the difference of demographics and influencing factors of motivations between whole blood donors and plasmapheresis donors, so as to provide scientific reference for effective recruitment strategy. M...
Analysis of risk factors in blood donors with confirmed positive syphilis
Objective To study the risk factors of blood donors confirmed to be positive for syphilis, so as to avoid highrisk groups, guide the recruitment of blood donors and improve blood safety. Methods From September 2021 to Au...
Establishment of a method for detecting complement C3d-sensitized platelets
[Objective] To establish a detection method for complement C3d-sensitized platelets. [Methods] Parallel detection of the same platelet sample under conditions of complement C3d sensitization and non-sensitization was con...
Diagnostic analysis and clinical treatment strategies for patients with hyperhemolytic syndrome: a case report
Objective To analyze a case of hyperhemolytic syndrome(HHS) and explore the laboratory diagnostic method and clinical treatment strategy. Methods Serological tests such as blood typing, direct antiglobulin test(DAT), ant...