INHIBITION OF E.CANCEROGENUS MAB-1 β -LACTAMASE BY SYNERGISTIC FORMULATION: AN IN VITRO HERBAL SHOTGUN STUDY
Journal Title: International Journal of Engineering Sciences & Research Technology - Year 30, Vol 5, Issue 7
Abstract
β-lactamases are increasing worldwide speedily by creating problem to currently available UTI therapies and hence there is an urgent need of research to find the effective therapy. In the present study we propose to develop drug formulation for the reversal of drug resistance in MDR E.cancerogenus MAB-1 by using bioenhancer property of isolated different phytochemicals (lead compounds). β-lactamase enzyme of E.cancerogenus MAB-1 was produced and purified up to 92.5 fold, by using Ion-Exchange chromatography(DEAE-Cellulose -52) techniques with 80.23 U/mg of specific activity. Iso-electric focusing and Sodium Dodecyl Sulfate –Polyacrylamide gel electrophoresis (SDS-PAGE) study revealed enzyme with PI value 8.5 with Molecular weight ~6.5 KDa. Threonine (9.27 ppm) and Serine (3.94 ppm) were detected in highest quantity when analyzed by GC-MS technique. Organic and Water extracts of selected plants were investigated for GC-MS, IR, NMR analysis for the isolation of pure lead compounds i.e. Theobromine, Myricetin, Gallic acid, Ellagic acid, Kaempferol and Genistein. In vitro Enterobacter cancerogenus MAB-1 β-lactamase inhibition by Dug-phytochemical combinations was studied by Checker board and Time-Kill curve at their ½ to 1/32 MIC values. The Theobromine+ Cefaclor synergy inhibited β-lactamases by 91%, Myricetin +Cefaclore (90%), Genistein+Cefaclore (92%), Genistein + Cefotaxime(90%), Genistein+Penicillin (91%) , Ellagic acid+Cefaclor (87%), Kaempferol+Ceftriaxone(87%), Kaempferol+Cefaclor (90%) and Clavulanic Acid (96%) Competitively, Gallic acid+Cefotaxime (92%) Non-Competitively. Thus the resulting data would be helpful to pick up the most effective and suitable combination therapy for further research in the area of drug development against UTI caused by E.cancerogenus MAB-1 or any member of Enterobacteriaceae family.
Authors and Affiliations
Rashmi M Bhande*
Keywords
Related Articles
- EP ID EP112452
- DOI 10.5281/zenodo.56972
- Views 91
- Downloads 0
A NOVEL APPROACH TO DETECT EQUIVALENT MUTANTS USING CONTROL FLOW GRAPH
Software testing verifies and validates software systems. Mutation testing is a testing procedure where mutants are created by seeding fault to the code. Test cases are exercised on these mutants to measure the ade...
COMPARISION OF ACADEMIC SCORES OF SPORTSMEN AND NON SPORTSMEN STUDENTS OF ENGINEERING COLLEGES
Physical Education is an integral part of the total education process. A field of endeavour that has its aim in the development of physically, mentally, emotionally and socially fit citizens through the medium...
[i][/i] NON-LINEAR PUSHOVER ANALYSIS OF RCC BUILDING WITH BASE ISOLATION SYSTEM
Buildings are designed as per the code regulations meeting all specific requirements of code and assuming a linear elastic behaviour for the structural members. Moreover, it is also necessary to know the behav...
FAULT ANALYSIS ON TRANSMISSION LINES USING ARTIFICIAL NEURAL NETWORK
The transmission line among the other electrical power technique component suffers from unexpected failure due to various random causes. The transmission line is quite large as it is open in the environment. The fault o...
Optimal Placement of Relay Nodes in Multi-pair Co-Operative Network to Increase System Capacity in WSN
With the advancement of technology the usage of wireless sensor network are increasing day by day. There are two types of sensor networks: Single tiered and multi-tiered [5]. In single tired sensor network there i...