Inhibition of K562 cell proliferation by cartilage polysaccharide with different relative molecular mass
Journal Title: Science Paper Online - Year 2009, Vol 4, Issue 3
Abstract
In order to study the proliferation of chronic myegenous leukemia(K562) treated by different relative molecular mass cartilage polysaccharide(sc-PS), cartilage extract was precipitated by ethanol with different concentration. Different relative molecular mass polysaccharides were collected. Then concentration and molecular mass of them were studied by SDS-PAGE; MTT method was used to test the proliferation of cells treated by sc-PS, and agarose gel electrophoresis was used to detect the characteristic of DNA. The results show that the growth of K562 cells is inhibited obviously by sc-PS, which in a dose-dependent, DNA ladder could be observed apparently in agarose gel electrophoresis.
Authors and Affiliations
Ning YANG, Anjun LIU, Wei Song
Keywords
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