Inhibition of Pro-Inflammatory Cytokine TNF-α by Boerhaavia diffusa (L.) in Liposaccharide stimulated Human THP-1 cells
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 6
Abstract
The production and release of proinflammatory cytokines such as Tumor necrotic factor-α (TNF- α), Interleukin-1β and Iinterleukin-6 from monocytes/macrophages play critical role in the pathogenesis of chronic inflammatory diseases and immune response. Boerhaavia diffusa L is a medicinal plant in the Indian traditional system known as Rasayana (Indigenous plants with immunomodulatory activity) and an important source of immunomodulators. In present study, the antiinflammatory activity of herbal immune modulator Boerhaavia diffusa L was studied by evaluating the inhibition of Lipopolysaccharide stimulated TNF-α secretion using human Monocytic THP-1 cells. Differentiation of the human monocytic cell THP-1 into macrophage-like cells was induced by exposure of the cells to Phorbol Myristate Acetate. When cells were pre-incubated with Methanolic extract of Boerhaavia diffusa L for 4 hrs and then stimulated with lipopolysaccharide for 12 hrs, secretion of tumor necrosis factor- α was significantly decreased compared to control. The present study has shown that the Boerhaavia diffusa L inhibits the production and release of proinflammatory cytokines, TNF-α from monocytes/macrophages which play critical role in the pathogenesis of chronic inflammatory diseases and hence this could be the probable mechanism, by which this immunomodulatory herbal plant extracts possess anti-inflammatory activity.
Authors and Affiliations
Jay H. Shah| Faculty of Pharmacy, Hemchandracharya North Gujarat University, Patan, India, I. S. Anand| Faculty of Pharmacy, Hemchandracharya North Gujarat University, Patan, India, K. K. Shah| Faculty of Pharmacy, Hemchandracharya North Gujarat University, Patan, India
Keywords
Related Articles
- EP ID EP16047
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(6).2820-2
- Views 353
- Downloads 24
Formulation and characterization of mouth dissolving mucoadhesive buccal film of Zolmitriptan
Fast dissolving buccal film drug delivery system is an alternative to tablets, capsules, and syrups for pediatric and geriatric patients who experience in difficulties of swallowing traditional oral solid dosage forms....
A validated analytical method for the estimation of Oxetacaine from its pharmaceutical formulation by RP-HPLC
OBJECTIVE: The aim of the current study was to develop an analytical procedure for the determination and quantification of Oxetacaine, from its marketed formulation which is simple, precise, accurate and a validated, r...
STUDY ON EFFECTS OF CARBAMAZEPINE AND PHENYTOIN ON DEVELOPMENT OF CHICK EMBRYO
The knowledge about the Teratogenic drugs is crucial while prescribing medications to the pregnant women. Treating epileptic pregnant women is always challenge as most of antiepileptic drugs are potent teratogens and th...
Evaluation of Synergistic Anti-microbial efficacy of plant extracts and their formulation as Topical Agents
The objective of the present study was to evaluate the antimicrobial activity of Cassia fistula, Ficusreligiosa, Milletiapinnataand Wendlandiathyrsoideaand to check the synergistic efficacy of these extracts when combi...
Formulation and evaluation of Phytosomesyrup for Hepatoprotective activity of Clerodendroninfortunatum Linn., root extract
The root portions of the Clerodendroninfortunatum Linn. roots extracted with ethanol by cold maceration process. The extract was vacuum dried and subjected to phytochemical screening for the detection of various phyto...