Investigating the In-vivo Antiplasmodial Properties of Aqueous Extract of Moringa oleifera Lam (Moringaceae) Leaves
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 5, Issue 6
Abstract
Aim: The study investigated Moringa oleifera Lam (Moringaceae), for its in-vivo antiplasmodial properties, using a murine model involving Plasmodium berghei. Study Design: Experimental. Places of Study and Duration: Department of Pharmaceutical Chemistry, KNUST, Department of Immunology, NMIMR and Department of Animal Experimentation, NMIMR, May – November, 2013. Methodology: Phytochemical investigation was conducted on powdered plant material and aqueous leaf extract (ML), to determine the presence of secondary metabolites using standard methods. Using 4-day suppressive and 7-day curative tests respectively, ML at 250-1000 mg/kg, was evaluated for in-vivo antiplasmodial activity in P. berghei infected male ICR mice (25-30 g), using Artemether-Lumefantrine (A/L) at 4 mg/kg as reference drug. The effect of ML on the body weights and the survival of the mice, as well as the effect on improvement in clinical signs was also monitored. Results: ML extract was found to contain alkaloids, saponins, flavonoids and tannins. The extracts (250-1000 mg/kg) produced significant reduction in parasitemia in the four-day suppressive test (F6,49 = 4.309; p =.0014). However, 250 mg/kg (69.31%; p< .001) and 500 mg/kg (77.26%; p< .001) extracts exhibited relatively higher activities compared to 750 mg/kg (25.28%; p< .001) and 1000 mg/kg (07.12%; p> .05). In the curative test, similar results were obtained with significant parasitemia reduction for 250 mg/kg (AUC = 52.52±6.732; p< .01) and 500 mg/kg (AUC = 49.62 ± 3.804; p< .01) compared to the positive control group (AUC = 101.3±14.32). Piloerection, lethargy and decreased locomotion were observed to be progressive in all infected experimental groups except A/L. Survival data showed that, although 750 mg/kg and 1000 mg/kg groups recorded relatively higher mortalities, statistical analysis didn’t indicate significant difference. Conclusion: ML extract demonstrated in-vivo antiplasmodial activity; probably due to the alkaloids, saponins, flavonoids and tannins in its composition.
Authors and Affiliations
Emmanuel Orman, Phyllis Addo, Michael Fokuo Ofori, Reimmel Kwame Adosraku
Keywords
Related Articles
- EP ID EP344458
- DOI 10.9734/BJPR/2015/15714
- Views 96
- Downloads 0
Gender Variation Studies in Dermatoglyphic Patterns (Level 2 Details) of the Ikwerre Ethnic Group in Rivers State, Nigeria
Background to the Study: Dermatoglyphics have had several definitions but each boils down to a simple sentence ‘act, process or science of studying ridge patterns on the skin of the palm, fingers, foot and toes of primat...
An Observational Prospective Study on Prevalence and Monitoring of Adverse Drug Reactions in Tertiary Care Teaching Hospital
Objective: To study the prevalence of ADRs in the in-patient departments of General Medicine, Pediatrics, Dermatology at tertiary care hospital. Materials and Methods: An Observational Prospective Study was designed from...
Effect of Sub acute Exposure to Telfairia occidentalis Root, Pod and Stem Extracts on Some Liver and Renal Function Parameters in Rats
Aim: This study assessed the effect of twenty eight (28)-day exposure to aqueous extract of Telfairia occidentalis root, pod and stem on the liver and kidney of Wistar rats. Methodology: Sixty eight (68) wistar rats wer...
Cytotoxicity of Enterolobium timbouva Plant Extract and Its Isolated Pure Compounds
Aims: The present study aimed to evaluate the cytotoxic activities of the aqueous alcohol extract of Enterolobium timbouva leaves as well as its isolated pure compounds. Place and Duration of Study: Department of Pharma...
Depression, Anxiety and Quality of Life among Chronic Low Back-leg Pain Patients with or without Neuropathic Pain
Aims: To investigate depression, anxiety and quality of life among chronic low back-leg pain patients with or without neuropathic pain. Place and Duration of Study: Ankara Numune Education and Research Hospital Departme...