Investigation of blaKPC Gene by PCR in Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae Clinical Isolates in a Tertiary Care Hospital in Turkey
Journal Title: Mediterranean Journal of Infection, Microbes and Antimicrobials - Year 2019, Vol 8, Issue 1
Abstract
Introduction: One of the most common mechanisms in the development of carbapenem resistance is acquiring carbapenemases. A new and different carbapenemase, Klebsiella pneumoniae carbapenemase (KPC), has recently emerged in Turkey. Klebsiella pneumoniae carbapenemases are encoded by the blaKPC gene and can be transferred between different species. Reports showing the presence of blaKPC gene in isolates from Turkey are limited. The current study aimed to investigate the presence of blaKPC gene in carbapenem resistant K. pneumoniae and Escherichia coli isolates in a tertiary care hospital in Turkey. Materials and Methods: Patient samples from various clinical units in Adana Numune Training and Research Hospital between November 2013 and October 2014 were sent to our laboratory at the same hospital. K. pneumoniae and E. coli strains isolated from different clinical samples were identified using both conventional methods and the VITEK® 2.0 automated identification system (bioMérieux, France). Imipenem-cilastatin, meropenem, and ertapenem susceptibility was analyzed using a VITEK® 2.0 antibiotic susceptibility testing system (bioMérieux, France). The presence of blaKPC gene was investigated by polymerase chain reaction. Results: A total of 49 resistant K. pneumoniae and 33 E. coli isolates were included in the study. Most were isolated from urine specimens. Among the carbapenems we tested, highest resistance rates were to ertapenem (98.0% in K. pneumoniae and 84.8% in E. coli isolates). One K. pneumoniae isolate was found positive for the blaKPC gene. Conclusion: According to our literature survey, this study is among the first reports from Turkey presenting the isolation of KPC-producing K. pneumoniae. This finding indicates the need to monitor carbapenem resistance arising from blaKPC, which may spread horizontally. Moreover, surveillance of antibiotic sensitivity rates and observation of regional differences will be useful guidelines in determining infection control and antimicrobial use management policies.
Authors and Affiliations
Gonca ÖZYAZICI, Esra ÖZKAYA, Havva KAYA, Sabriye KOCATÜRK SEL, Hasan ALTINBAŞAK, Fesem BAŞARI, Bedia Mutay SUNTUR, Celal Kurtuluş BURUK
Keywords
Related Articles
- EP ID EP689264
- DOI 10.4274/mjima.galenos.2019.2019.3
- Views 176
- Downloads 0
Progressive Multifocal Leukoencephalopathy Presenting with Bilateral Myoclonus: A Case Report
Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system (CNS) caused by John Cunningham virus lytic infection of the oligodendrocytes, the myelin-producing cells in the C...
Posttraumatic Septic Arthritis Due to Serratia marcescens: Case Report and Review of Literature
Septic arthritis is classified among the acute emergencies of joint diseases. Gram-positive cocci are responsible in 75-80% of the cases, while Gram-negative bacilli may be the etiological agents in case of compromised i...
Distribution of AdeABC Efflux System Genes in Acinetobacter baumannii Isolated from Blood Cultures of Hospitalized Patients and Their Relationship with Carbapenem and Aminoglycoside Resistance
Introduction: The increasing emergence of multidrug-resistant (MDR) Acinetobacter infections has become a significant challenge for physicians and clinical microbiologists owing to the difficulties arising during therapy...
Osteoarticular Involvement and Inadequate Treatment of Brucellosis are Related to Relapse
Introduction: The goal of treatment in brucellosis is to control symptoms to prevent the development of complications and relapse of infection. The aim of this study was to evaluate the risk factors for relapse in brucel...
What to Know About Travel Related Infections
Travel is as old as human history. People travel for purposes such as migration, commerce, education, missionary activity, and entertainment. Historically, these human movements gave rise to geographical discoveries, tra...