Investigation of Carbapenemase Genes and Clonal Relationship in Carbapenem Resistant Klebsiella pneumoniae Strains
Journal Title: Bezmiâlem Science - Year 2019, Vol 7, Issue 3
Abstract
Objective: Resistant Gram-negative bacteria isolated from health-related infections are a worldwide problem. Increasing frequency of infections particularly caused by Enterobacteriaceae producing expanded spectrum beta lactamase, leads to the use of more carbapenem group antibiotics which, in turn, leads to bacterial resistance. In this study, we aimed to evaluate carbapenem resistance in Klebsiella pneumoniae (K. pneumoniae) isolates, the mechanisms causing this resistance and the clonal relationship between these isolates. Methods: Ninety one K. pneumoniae strains isolated from clinical samples obtained in our laboratory were included to the study. The identification of the bacteria was performed with Matriks assisted laser desorption ionization time of flight mass spectrometry (bioMérieux, Marcy-I’Étoile, France) and antimicrobial susceptibility with VITEK-2 (bioMérieux), and the carbapenem resistance was confirmed by ertapenem E-test (bioMérieux). Reverse transcription polymerase chain reaction method was used for the investigation of genes causing carbapenemase production (blaOXA-48, blaNDM-1, blaKPC, blaIMP, blaVIM-1). The clonal relationship between isolates was investigated by pulsed-field jel elektroforez. Results: In carbapenem resistant isolates, blaOXA-48 positivity was found to be 55%, blaNDM-1 positivity 37.4%, blaKPC and blaVIM-1 positivity 1.1%. A total of 10 isolates was identified with different resistance genes. In 73 of the isolates included in the study, the clonal relationship was examined, and 16 different groups were identified. Twenty isolates were not clonally associated with any other isolates. The most common resistance mechanism causing the carbapenem resistance was blaOXA-48 gene that is known to be endemic in Turkey. Conclusion: As a result, the carbapenem resistance that we found as 3.13% in our study is similar to the rates obtained in other studies performed in our country which indicates that this resistance is not at a high level yet in our country. However, the ability of carbapenem resistance genes to spread between strains can be a major problem in the near future. Molecular methods are gold standard in carbapenemase detection, but because of having high cost they can not be used in laboratories routinely. Modified Hodge test or carbapenemase inactivation test are alternative tests with low costs that can be used in the determination of carbapenemase.
Authors and Affiliations
Mustafa SAMASTI, Mücahide Esra KOÇOĞLU, İsmail DAVARCI, Haluk VAHABOĞLU, Hülya ÇAŞKURLU
Keywords
Related Articles
- EP ID EP50744
- DOI 10.14235/bas.galenos.2018.2675
- Views 237
- Downloads 0
Retrospective Analysis of AMATEM Patients Treated in Psychiatry Inpatient Unit of Adiyaman Training and Research Hospital: Data for 2018
Objective: The prevalence of substance use disorder (SUD) is increasing in the world and in our country. The most important centres in the treatment of SUD in Turkey are Alcohol and Drug Addiction Treatment and Research...
Impact of UCP2 -866G/A Variant on Smoking Risk
Objective: Mitochondria are multifunctional and dynamic organelles found in cells. Nicotine is a natural alkaloid found in the tobacco plant and has been well studied as a component of cigarette smoke. It has also been r...
Carbonic Anhydrase Isoenzymes I and II Inhibition Potentials of Leiotulus dasyanthus (K. Koch) Pimenov&Ostr. and Ferulago pauciradiata Boiss.&Heldr. (Apiaceae)
Objective: "Members of the Apiaceae family have many biological and pharmacological effects like anticancer, antidiabetic, antioxidant, anti-inflammatory, anti-hypertensive, anticoagulant, antimicrobial, and anticholines...
The Effect of Simulation-based Training on the Self-confidence and Self-satisfaction of Nursing Students Dealing with Patients under Isolation
Objective: Simulation is an innovative education model that is highly effective in nursing training. The implementation of innovative training programmes on the importance of infection control increases the awareness of...
Comparison of Small-diameter-hole and Traditional Microfracture in Cartilage Repair and the Effect of Adding a Hyaluronic Acid-based Acellular Matrix Scaffold: An Animal Study
Objective: Since, there is no standardized technique for the treatment of focal cartilage defects that can recreate original cartilage tissue; researchers continue to explore and evaluate various treatment modalities. Th...