Investigation of the Different Ethnopharmacological Activity of Fractional Root Extracts of Mussaenda roxburghii in in vitro Model
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 10, Issue 2
Abstract
Purpose: With this study, we wanted to reveal that the root extract of M. roxburghii has thrombolytic activity or not and targeted to estimate its toxicity, potentiality to inhibit inflammation by the inhibition of protein denaturation. Methods: In vitro thrombolytic assay was used in the purpose of clot lysis capacity measurement of fractional crude extracts of M. roxburghii. We took streptokinase as a positive control. Brine shrimp lethality bioassay was performed to measure the toxic potentiality of the plant extracts. The in vitro anti-inflammatory assay was designed using egg protein model to check the percent protection rate of protein denaturation. Results: In the thrombolytic assay, ethyl acetate extract of M. roxburghii showed significant (p <0.0001) clot lysis activity with 48.85±7.38%. The positive control Streptokinase results 76.90±9.01% and negative control water results very little, 7.55±3.33%. The apoptotic activity study showed good result with EC50 of 32.46 µg/ml with significant limit of <0.002. In in vitro anti-inflammatory study the ethyl acetate extract of M. roxburghii showed highest percent of inhibition with 48.43±2.23% (p<0.001). Conclusions: From our study outcome, the ethyl acetate and dichlormethane extracts of root of M. roxburghii have good thrombolytic, anticancer and anti-inflammatory activity. Hence, further study is needed to identify the phytochemicals incorporated with thrombolytic activity, the new chemicals may aid in the improvement of the treatment of atherosclerosis or embolism like diseases. We should also take result of pain inhibition and anti-cancer study, as well.
Authors and Affiliations
Farzana Akther Sumi, Prawej Ansari, Biswajit Sikder, Anaytulla . , Nadia Akter Zhumur, Mustafe Khalid Mohamed, Sabbir Anwar, Mitali Debnath, Rokibul Hasan
Keywords
Related Articles
- EP ID EP350073
- DOI 10.9734/EJMP/2015/19315
- Views 102
- Downloads 0
Antiplasmodial Potential of the Ethanol Leaf Extract of Triclisia macrophylla and Its Fractions
Different plant extracts are used in Africa by the population as treatment for malaria. The antiplasmodial effect of the ethanol extract and fractions of Triclisia macrophylla was thus evaluated using Swiss albino mice 1...
In vitro Evaluation of Membrane Stabilizing Potential of Selected Bryophyte Species
Aims: The purpose of the current study was to investigate the possible anti-inflammatory properties of the three bryophytes species: Archidium ohioense, Bryum coronatum and Racophilum africanum with a view to utilizing t...
Antiplasmodial Potential of Traditional Medicinal Plant Thlaspi arvense
Aim: Antiplasmodial potential of traditional medicinal plant Thlaspi arvense against Plasmodium falciparum in vitro has been evaluated. Cytotoxicity of plant extract against HeLa cell lines and normal fibroblasts has als...
Qualitative Characterization of Solvent and Cooked Extracts of Tribulus terrestris L. Fruit
Currently there has been an increased attention globally to identify antioxidant compound that are pharmacologically potent and have low or no side effects. As plants are source of natural antioxidants, much concentratio...
Total Antioxidants Status, Selenium Concentration, Catalase and Glutathione Peroxidase Activities in Rabbits Fed a High-Garlic Diet
Aim: Results from previous studies on the effects of intake of garlic on the body antioxidant system appear inconclusive. This study measured glutathione peroxidase (GPx) and catalase (CAT) activities, Total Antioxidant...