Invitro Regeneration of a Threatened Medicinal Plant Phyllodium Pulchellum L. Desv.
Journal Title: International Journal of Botany and Research (IJBR) - Year 2017, Vol 7, Issue 4
Abstract
An efficient in vitro regeneration protocol was developed Phyllodium pulchellum (L.) Desv. A threatened medicinal plant. The plants were regeneration from shoot tip and nodal explants. The highest number of shoots per explant was obtained on the medium supplemented with 1.5 mg/l BAP + IAA 0.5 mg/l from shoot tip explants (4.30±0.40) and nodal explants (4.06±0.31). The elongated shoots were successfully rooted on MS with NAA (α- naphthalene acetic acid) or IBA; 0.75 mg/l of NAA provided better response for rhizogenesis among them. For regenerated shoots rooting, MS medium supplemented with 0.75 mg/l NAA (α- naphthalene acetic acid) was most effective with maximum number (4.50±0.36 roots per plantlet) and length (2.76±0.15 cm per root) of roots respectively. Regenerated plantlets were successfully transferred into pots with soil and over 90% of them grew into healthy mature plants. This is the first report of direct organogenesis in P. Pulchellum with significantly high plantlet regeneration frequency.
Authors and Affiliations
Gopal Velmurugan, Subramaniam Parvathi Anand
Keywords
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