Isolation and characterization of dipropyl-, S-propyl ester from Exiguobacterium mexicanum (MSSRFS9) against larvae of malaria and dengue vectors
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2016, Vol 6, Issue 6
Abstract
Objective: To evaluate the insecticidal property of the compound dipropyl-, S-propyl ester extracted from the Gram-positive rhizosphere bacterium against the fourth instars larvae of malaria vector, Anopheles culicifacies (An. culicifacies) and dengue vector, Aedes albopictus (Ae. albopictus). Methods: Bacteria were taxonomically identified as Exiguobacterium mexicanum (E. mexicanum) and different crude solvent extracts were examined for its larvicidal potential against mosquito vectors. The crude extract was eluted in silica gel column chromatography and the pure compound was identified. Results: The highest larvicidal activity against An. culicifacies was exhibited by ethyl acetate extract. The bacterium of E. mexicanum extract at 150, 300, 450, 600, 750 mg/L caused against both vector mosquito species. Hexane, chloroform, acetone, methanol and ethyl acetate caused moderate considerable mortality. The highest larval mortality was observed in ethyl acetate extract. Conclusions: The results of the bacterial extract of E. mexicanum are promising as potential larvicidal compound against the mosquito vectors (An. culicifacies and Ae. albopictus). Therefore, this study provides first report on the larvicidal activity with dipropyl-, S-propyl ester compound from India.
Comparison of plasmid and chromosomal omp1 gene-based PCR and two DNA extraction methods for diagnosing Chlamydia trachomatis in endocervical swab samples
Objective: To evaluate the effectiveness of boiling and proteolytic DNA extraction methods and also to compare the sensitivity of plasmid polymerase chain reaction (PCR) and chromosomal omp1 gene PCR for genital Chlamydi...
Modeling of dengue occurrences early warning involving temperature and rainfall factors
Objective: To understand dengue transmission process and its vector dynamics and to develop early warning model of dengue occurrences based on mosquito population and host-vector threshold values considering temperature...
Incidence of Listeria species in bovine, ovine, caprine, camel and water buffalo milk using cultural method and the PCR assay
Objective: To determine the prevalence rate of Listeria species in bovine, ovine, caprine, camel and water buffalo milk in Iran. Methods: From September 2010 to December 2011 a total of 260 bulk milk samples including 85...
In vitro antitrypanosomal activity, antioxidant property and phytochemical constituents of aqueous extracts of nine Nigerian medicinal plants
Objective: To study the in vitro antitrypanosomal activity, antioxidant property and phytochemical constituents of aqueous extracts of nine Nigerian medicinal plants. Methods: In vitro antitrypanosomal activity test was...
Diabetes induced testicular dysfunction amelioration by ethyl acetate fraction of hydromethanolic extract of root of Musa paradisiaca L. in streptozotocin-induced diabetic rat
Objective: To investigate the diabetic therapeutic potentiality and antioxidative efficacy of ethyl acetate fraction of hydro-methanol (40:60) extract of root of Musa paradisiaca Lam. (Musaceae) in streptozotocin-induced...