Isolation and Characterization of Polyaromatic Hydrocarbons Degrading Bacteria from Compost Leachate
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 2
Abstract
Polycyclic aromatic hydrocarbon (PAHs) degrading bacteria were isolated from compost leachate (CL) that collected from a composting site located in central Scotland, UK. Isolation was carried out by enrichment using phenanthrene (PHR), Pyrene (PYR) and Benzo(a)pyrene (BaP) as the sole source of carbon and energy. The isolates were characterized using a variety of phenotypic, morphologic and molecular properties. Six different isolates were collected based on the difference in morphological and biochemical tests by using API 20E and API NE, also for their efficiency in PAHs utilization. The 16S rDNA sequence analysis confirmed the results of biochemical identification, as both of biochemical and molecular identification of the isolates assigned them to Bacillus licheniformis, Pseudomonas aeruginosa, Alcaligenes faecalis, Serratia marcescens, Enterobacter cloacae and Providenicia rettgeri which were identiï¬ed as the prominent PAHs-utilizers isolated from (CL). This study indicates that the (CL) samples contain a diverse population of PAHs-degrading bacteria and the use of (CL) may have a potential for bioremediation of PAHs contaminated sites.
Authors and Affiliations
Said M. Badr El- Din, Tarek A Moussa, Hassan Moawad, Omaima A Sharaf
Keywords
Related Articles
- EP ID EP654423
- DOI 10.24297/jab.v5i2.3772
- Views 192
- Downloads 0
A histo-morphometric study on the developing gastrointestinal tract of the axolotl, Ambystoma mexicanum
The histological structure of the developing gastrointestinal tract (GIT) of the axolotl, Ambystoma mexicanum, was investigated during embryonic, larval and juvenile stages. The earliest histological events witnessed the...
In vivo and in vitroevaluation of the Acrocomia aculeatapulp oil - lipid profile and oxidative stress
Acrocomia aculeata is a plant species with high levels of antioxidant compounds, rich in carotenoids with high bioavailability and monounsaturated fatty acids. This study was designed to investigate the biological effect...
Biochemical Changes Induced by Gamma Irradiation in the Ground Beetle Blaps polycresta
The exposure to low-dose gamma radiation from natural sources is a continuing and inescapable feature and it is common in certain occupations. The present study was designed to investigate the effects of low-dose gamma r...
EMBRYONIC DEVELOPMENT OF MOLGULA MANHATTENSIS (DE KAY, 1843) WITH EMPHASIS ON THE EFFECT OF SOME BIOACTIVE INDUCERS AND HSP 90 ON LARVAL METAMORPHOSIS
Specimens of Molgula manhattensis (De Kay, 1843) were collected during March to June (2013) along the northern estruarine harbour of the Arabian Gulf - Saudi Arabia. Sperm and eggs were sucked from gonoducts and placed s...
Correlation of serum Nitric Oxide and hs-CRP in non-smoker and non-diabetic patients with Coronary Artery Disease
Back ground: The nitric oxide (NO) decrease and inflammation are cooperative events involved in atherosclerosis development. In the present study we assessed the correlation of NO with high sensitive c- reactive protein...