Isolation and characterization of thermostable lipases from themophilic bacteria isolated from hot water spring
Journal Title: INTERNATIONAL JOURNAL OF RECENT TRENDS IN SCIENCE AND TECHNOLOGY - Year 2018, Vol 26, Issue 2
Abstract
Twelve isolates were screened for lipases production from hot water spring, Vajreshwari, Thane, Maharashtra on a Nutrient agar and nutrient broth medium consisting of olive oil. The isolate showed highest lipase activity was VIII which later was identified as phingomonas paucimobilis. The effect of pH, temperature and substrate concentration was studied on partially purified lipases by ammonium sulphate precipitation from Sphingomonas aucimobilis. The partially purified lipase had maximal activity at pH of 7.The optimum temperature for the hydrolysis of olive oil at 60°C.The lipases has ability to hydrolyse used fried oil. The transesterification activity of lipases was used to synthesis biodiesel. The analysis of synthesized biodiesel was performed by FTIR( Fourier transform infrared spectroscopy) and free fatty acid content.
Authors and Affiliations
R J Pungle, S G Sapkal, B D Vyawahare, P R Bhandwalkar, S B Bedwal
Keywords
Related Articles
- EP ID EP365971
- DOI -
- Views 97
- Downloads 0
Hepatic hydatid cyst – Incidence and prevalence
This is a prospective, single centered based observational study of 30 patients with hydatid cyst of liver included the patients admitted in the Department of General Surgery In M.G.M Medical College and L.S,K Hospital l...
To Study the Chemotherapeutic Effect of Doxorubicin When Combined With Aspirin and Clopidogrel in Ehrlich Ascites Carcinoma Induced Adult Wistar Rats
Objective: To determine whether there is an enhanced chemotherapeutic effect of Doxorubicin when combined with Aspirin and Clopidogrel (anti platelet drugs) in Ehrlich Ascites Carcinoma induced adult wistar rats.Methodol...
Comparative study of polypropylene hernia system against lichtenstein for hernia repair with respect to surgical parameters
Introduction: Inguinal hernia repair is the most common general surgery procedure, and several hundred thousand are performed every year in the United States. Countless studies have been done in attempts to improve outco...
Morrissey’s Cough Test - A Student Friendly Modification
Morrissey’s cough test is poorly described in recent text books of surgery and the students have difficulty in recognizing the response to cough. Here we suggest a simple modification of the test which makes it easier fo...
Prevalence of Reproductive Tract Infections and its Association with Sociodemographic Profile among Women in Reproductive Age
Background Reproductive tract infections (RTIs) cause considerable morbidity among women of reproductive age worldwide. Any morbidity in this age group will interfere with overall socio economic development of community....