Isolation and Identification of Bacterial Contaminants from Door Handles of Public Toilets in Federal University Dutse, Jigawa State- Nigeria
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 5
Abstract
Public contact surfaces such as door handles frequently touched with hands can act as vehicles of diseases transmission. The aim of this study was to isolate, identify and evaluate the presence or absence of bacterial contaminants present on the door handles of public toilet in the Federal University, Dutse, Jigawa State, north-western Nigeria in order to take the necessary remedial measures. The study analyzed eighty-five (85) swab samples from public toilets door handles within the university campus. Sterile swabs were firmly pressed on the various door handles; up/down, left/right and diagonally. Samples collected were cultured and incubated at 370C for 24 hours. Isolation and identification of bacteria was done using standard microbiological procedures. A total of one hundred and fifteen (115) colonies were isolated in this study based on cultural, morphological and biochemical characteristics. Frequency distribution of the isolates showed that Staphylococcus aureus were 44(38.3%), Bacillus species 26(22.6%), Escherichia coli 16(13.9%), Micrococcus spp 13(11.3%), Salmonella spp 10(8.7%) and Klebsiella spp 6(5.2%). The level of contamination varies depending on the traffic exposure and the environment. This means that it is necessary to practice good personal hygiene through hand washing and use of hand sanitizer as well as daily washing and cleaning of toilets to reduce the incidence of microbial transmission.
Authors and Affiliations
1Bashir, S. F, Muhammad, H, Sani, N . M, 2Kawo, A.H
Keywords
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- EP ID EP386650
- DOI 10.9790/3008-1105045357
- Views 76
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