Isolation And Identification Of Halophilic Bacteria Producing Halotolerant Protease
Journal Title: Science Heritage Journal | Galeri Warisan Sains (GWS) - Year 2017, Vol 1, Issue 1
Abstract
Halotolerant proteases are known as one of the important groups of enzymes that have been used widely in various industries. However, high production cost of proteases in term of energy used for sterilization and high risk of microbial contamination during fermentation become the obstacles during upstream processing. Moreover, proteases that capable to withstand with harsh conditions of salinity, temperature and pH are required in industry. Therefore, this study presents newly isolated halophilic bacteria producing halotolerant proteases. Halophilic bacteria were isolated from fermented fish sauce. Screening of the bacteria producing halotolerant protease enzymes was carried out by using skim milk salt agar containing 10% NaCl at pH 7 and incubated under aerobic condition at 37oC for 2 days. The selected isolates were identified based on their morphology followed by 16S rDNA gene sequence analysis. 40 colonies of halophiles bacteria were isolated, however, only 20 of them showing proteolytic activity. All of 20 isolates are motile and gram positive bacteria. From 20 isolates, only 6 were chosen for further analysis. B7 showed the highest proteolytic activity compared with others. Results of 16S rDNA gene sequence analysis showed 98% homology to Bacillus amyloliquefaciens subsp. plantarum strain FZB42. Therefore, B7 is identified as Bacillus amyloliquefaciens strain B7.
Authors and Affiliations
Nadiah Syuhada Abd Samad, Azura Amid, Dzun Noraini Jimat, Nurul Aqilah Ab. Shukor
Keywords
Related Articles
- EP ID EP255924
- DOI 10.26480/gws.01.2017.07.09
- Views 145
- Downloads 0
HIGH-QUALITY DNA FROM PEAT SOIL FOR METAGENOMIC STUDIES: A MINIREVIEW ON DNA EXTRACTION METHODS
The understanding of microorganism’s biodiversity in the peatland soil through non-cultivation based approach provide important inputs towards prediction of the ecosystem response towards the changing environment. The ch...
ANTIOXIDANT AND CYTOTOXIC EFFECTS OF CURCUMA MANGGA AND BOSENBERGIA ROTUNDA ETHANOLIC EXTRACTS ON MCF-7 CANCER CELL LINES
Curcuma mangga and Bosenbergia rotunda which belongs to Zingiberaceae family are largely distributed in South East Asian countries including Malaysia. These plants have been traditionally used for treatment of various di...
Red Pigment Production By Monascus Purpureus In Stirred-Drum Bioreactor
Several studies has been conducted to economically cultivate the Monascus sp. However, the potential of using stirred drum bioreactor in solid state fermentation (SSF) for Monascus sp. cultivation has been relatively und...
THE DAMPING LOSS PREVENTION RESEARCH ON AEROBICS SPECIAL SHOE MATERIALS BASED ON INTELLIGENT ANALYSIS
In recent years, with the people’s awareness of the changes in the fitness movement, aerobics has become one of the most popular sports. Although it is only a moderate intensity of sports items, but there are still likel...
Pcb biodegration using bacteria isolated from landfill leachate
Polychlorinated biphenyls, PCBs are toxic, persistent organic pollutants (POP) which are harmful to human and environment due to their lipophilic characteristic and not easily degraded in the environment. Biodegradation...