Isolation and Identification of Microorganisms from Crude oil Contaminated Soils of Dar es Salaam, Tanzania
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 8
Abstract
Aims: The aim of this study was to isolate and identify microorganisms in crude oil polluted soils in Eastern Tanzania. Study Design: Isolation and identification of microorganisms using conventional and molecular techniques. Place and Duration of Study: Department of Molecular Biology & Biotechnology, University of Dar es Salaam; October 2012 to June 2013. Methodology: Soils were collected from Mlalakua (ML), University of Dar es Salaam (UDSM) and Ubungo (Ub) local garages in Dar es Salaam, Tanzania. The selective isolation of microorganisms was done based on colony morphological appearance on agar plates and Gram reaction. Three isolates (one from each site) were subjected to molecular methods for the identification purposes. Phylogenetic analyses of bacterial 16S rRNA gene and fungal ITS region sequences were employed in this study to identify and determine the evolutionary relationships of the ML, Ub and UDSM isolates. Results: The 16S rRNA analysis revealed that the ML isolate belonged to the genus Klebsiella, while the ITS analysis on Ub and UDSM isolates assigned them to the genus Trichosporon and Candida, respectively. Conclusions: From these findings, further studies are recommended to assay for the biodegradative potentiality of the identified isolates for bioremediation of crude oil contaminated soils.
Authors and Affiliations
Anthony Manoni Mshandete, Peter Elias Kidibule, Eva Mathias Sosovele
Keywords
Related Articles
- EP ID EP237713
- DOI 10.9734/BBJ/2014/11780
- Views 119
- Downloads 0
Relationship of Genomic G+C Content between Phages/Plasmids and Their Hosts
Aims: We compared genomic G+C content between bacteriophages/plasmids and their host genomes of 46 species to reveal their relation. To examine the large deviation in the G+C content between bacteriophages and their host...
Genotyping and Nucleotide Sequences of Growth Hormone Releasing Hormone and Its Receptor Genes in Egyptian Buffalo
Aim: The hypothalamic hormone, growth hormone-releasing hormone, is the principal stimulator of pituitary growth hormone (GH) synthesis and secretion. GHRH and its receptor (GHRHR) provide important functions in the regu...
Isolation and Characterization of Cellulase Producing Bacteria from the Gut of Termites (Odontotermes and Heterotermes Species)
Aim: To identify, characterize and compare the cellulolytic potentials of strains isolated from the gut of Odontotermes and Heterotermes species. Methodology: Termites were collected, identified, surface sterilized and...
In vitro Antitheilerial Activity of Paluther (Artemether 80) Against Theileria lestoquardi
Aim: The aim of this study was to screen Artemether 80 for activity against Theileria lestoquardi (Apicomplexa: Theileridae) using buparvaquone as a standard drug. Study Design: In vitro study under laboratories conditio...
Molecular Differentiation of Five Quinoa (Chenopodium quinoa Willd.) Genotypes Using Inter-simple Sequence Repeat (ISSR) Markers
Knowledge of genetic diversity is one of the important tools used for genetic management of quinoa accessions for plant breeding. This research aimed to molecularly characterize five quinoa genotypes using ISSR markers t...