Isolation and Optimization of Lipid Production from Drechslera Sp. and Feasibility of using Orange Peel as a Substrate for Growth
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2018, Vol 7, Issue 0
Abstract
This study deals with isolation and optimization of soil origin fungus Drechslera sp. with valued unsaturated fatty acids using orange peel as a substrate. Accordingly, to maximize oil production, various parameters were optimized using 'one variable at a time' strategy followed by employing statistical designs (Plackett-Burman (PBD) & Response surface methodology (RSM)). The results showed that incubation time, FeSO4, pH and yeast extract were the most significant factors influencing lipid accumulation. Therefore, RSM employed to optimize their concentrations. The optimum parameter values were: incubation time 144 h, FeSO4 0.015 g/L, pH 8.5 and yeast extract 2 g/L. Under the optimized conditions, a total biomass of 12 g/L with a lipid content of 40.75 % (corresponding to a lipid yield of 4.89 g/L) was achieved. The fatty acid profile revealed production of 41.99 % of unsaturated fatty acids. The feasibility of utilizing orange peel as a sole carbon source proved that it represents a potentially valuable source of single cell oil (SCO) with oil yield of 3.65 g/L. Its FAME profile revealed an increment of unsaturated fatty acids up to 58.86% with desirable amounts of polyunsaturated fatty acids (PUFA) 33.31 %, this making it as ideal substrate for production of unsaturated fatty acids.
Authors and Affiliations
Hadeel Said El-Shall
Keywords
Related Articles
- EP ID EP653916
- DOI 10.24297/jbt.v7i1.7589
- Views 62
- Downloads 0
Genetic analysis of agronomical traits in recombinant inbred lines of cowpea (Vignaunguiculata(L.) Walp.)under two water regimes.
Single nucleotide polymorphism (SNP) markers were used to develop a genetic-linkage map and to identify QTLsinvolved in the genetic variation of agronomical traits in cowpeaunder two water regimes. A total of 1536 SNP Go...
Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571
Yacon is a perennial plant native of the Andesthat stores inulin-type polyfructans as reserve carbohydrates in its tubers. The enzymes that hydrolyze the inulin β-2,1 fructose linkages are called inulinases and are much...
Improving the anti-oxidation of glucose oxidase with computer-aided structure optimization
Glucose oxidase (GOD) can be inactivated by hydrogen peroxide (H2O2) produced during glucose oxidation; thus, H2O2 is a competitive inhibitor of GOD. In this mechanism, methionine (Met), a sulfur-containing amino acid, c...
Carica Papaya; A Paired and Alternative Therapy of Dengue Fever
The purpose f the study is to explore the prospective use of Carica papaya leaves extracts for Dengue fever that is a painful, debilitating mosquito-borne disease caused by dengue viruses. An estimated 390 million dengue...
Therapeutic effectiveness of Hydroxyapatite Nanoparticles and Pulsed Electromagnetic Field in Osteoporosis and Cancer
The emergence of nanotechnology has had a profound effect on many areas of healthcare and scientific research. Several studies reported the importance Hydroxyapetite Nanoparticles in the biomedical field in general, and...