ISOLATION AND PURIFICATION OF INTRACELLULAR CHITOSANOLYTIC ENZYMES OF Mucor circinelloides
Journal Title: Progress on Chemistry and Application of Chitin and its Derivatives - Year 2008, Vol 0, Issue
Abstract
Application of the authors’ new, two-step (bacitracin affinity chromatography and gel filtration on Sephadex G-100) method of purification of M. circinelloides intracellular enzymes gave the preparation with the relatively high activity of both endo-chitosanases and lipases. Our investigations indicate that this purified preparation contains two proteins with almost the same molecular masses and different activities. We have been trying to separate these proteins and purify them to homogeneity. We also intend to determine their 3 D structures and properties. However, the hitherto published results and our findings suggest that we may also identify the new oligomeric lipase, which efficiently depolymerizes chitozan.
Authors and Affiliations
Katarzyna Struszczyk, Mirosława Szczęsna-Antczak, Marta Walczak, Tadeusz Antczak
Keywords
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