Isolation, Identification and Antimicrobial Susceptibility Pattern of Escherichia Coli Isolated From Urine Samples at Tertiary Care Hospital
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 10
Abstract
Urinary tract infection (UTI) is the most common bacterial infection caused by both gram negative bacilli and gram positive cocci among which E. coli remains the leading uropathogens involving both men and women of all age groups. To isolate, identify and study the Antimicrobial Susceptibility Pattern of E. coli isolated from urinary tract infected patients of both sex of all age group. A sterile urine samples were collected from various clinical departments with suspected urinary tract infection and transported to central laboratory. The samples were subjected to direct microscopy, culture and biochemical tests for isolation of bacterial pathogens. Organisms were identified and Antimicrobial susceptibility testing was performed on Muller-Hinton agar by Kirby-Bauer disk diffusion method following CLSI (Central Laboratory Standard Institute) guidelines. A battery of antibiotics includes Gentamycin (10µg), Tobramycin (10µg), Ciprofloxacin (5µg), Ceftazidime (30µg), Piperacillin-Tazobactam (100/10µg), Ceftriaxone (30 µg), cefuroxime (30µg), Imipenem (10µg), Trimethoprim-sulfamethoxazole (1.25/23.75 µg), Ampicillin (10µg), Amoxicillin-clavulanic acid (20/10µg) and Nitrofurantoin (300 µg). A total of 486 urine samples were processed in central laboratory out of which 88 were isolated and identified as E. coli in both sex of all age groups. The E. coli isolates, were highly susceptible to Imipenem (95%), Piperacillin-Tazobactam (90%), Tobramycin (80%), Gentamicin (75%), nitrofurantoin 94%, with maximum resistance to Ampicillin (75%) and Amoxicillin-Clavulanic acid (68%). In this study the antimicrobial susceptibility to uropathogenic E.coli were studied and most classes of antibiotics were found to be sensitive.
Authors and Affiliations
Dr. Sathyavathy K, Dr. Kiran Madhusudhan B, Dr. Chitralekha Saikumar
Keywords
Related Articles
- EP ID EP478919
- DOI -
- Views 64
- Downloads 0
Role of Tramadol in Labor Analgesia
The aim of the present study to assess the efficacy and safety of intramuscular tramadol hydrochloride as an analgesic during labor. Its efficacy has compared with placebo group. This is a prospective study conducted for...
Evaluation of Risk for Type 2 Diabetes Mellitus in Medical Students Using Indian Diabetes Risk Score (IDRS)
Diabetes Mellitus (D.M.) refers to a group of common metabolic disorders that share the phenotype of hyperglycemia. The rising prevalence of type 2 D.M is closely associated with industrialization and socio economic deve...
Efficacy of 2% Rebamipide Eyedrops in Patients with Superior Limbic Keratoconjunctivitis
Abstract: Rebamipide, an oral mucosal protective agent approved in Japan for gastric mucosal disorders and gastritis has been recently introduced as an ophthalmic solution. Being a mucin secretagogue along with a goblet...
To Study the Clinical Profile of Meconium Aspiration Syndrome (MAS) in Neonates in Relation to Birth Weight, Gestational Age and Their Immediate Outcome
Study the clinical profile of meconium aspiration syndrome (MAS) in neonates, in relation to birth weight, gestational age and their immediate outcome. Prospective observational cross sectional study. All preterm, term a...
Economic Evaluation of PPI Prescriptions in Patients: A Cost Minimization Analysis
Abstract: PPI are the most commonly used drugs in clinical practice. Different PPIs do not have any important differences clinically. Reports have estimated that over 60% of all PPI prescriptions are generated from inpat...