ISOLATION, IDENTIFICATION AND SPECIATION OF CANDIDA SPECIES FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE TEACHING HOSPITAL IN KARNATAKA, INDIA
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2019, Vol 6, Issue 11
Abstract
BACKGROUND Candida species cause different clinical infections ranging from mucocutaneous infection to life threatening invasive diseases. They cause secondary infection in immunocompromised individuals. Candidiasis is a common fungal disease in humans. During the last decade, an increase in the prevalence of non-albicans candida species has been noted because of increased use of azoles. We aimed to isolate Candida species, find its frequency from various clinical samples and speciate Candida using chromogenic medium. MATERIALS AND METHODS A total of 100 Candida isolates from various clinical samples were included in the study. Colony on Sabouraud dextrose agar was processed to identify the Candida species using conventional methods. Simultaneously, they were processed for Candida speciation on commercially available CHROMagar (HiMedia, India). RESULTS In the present study, majority of isolates were from high vaginal swab (33%) followed by sputum (27%), urine (18%), pus from surgical sites and others constituted to 22%. Candida albicans (54%) was the most common candida species, followed by C. tropicalis (24%), C. krusei (14%), C. glabrata (7%) and C. dubliniensis (1%). CONCLUSION Candida albicans was the predominant species causing Candidiasis. Along with Candida albicans, non-albicans candida (NAC) spp. like C. tropicalis, C. krusei, C. glabrata, and C. dubliniensis are increasingly being isolated from clinical samples. CHROMagar is a simple, easy, rapid, reliable and inexpensive method for identification of candida species. Characterization of candida to species level is important for early treatment decisions and effective management as some NAC species are intrinsically resistant to antifungal agents. CHROMagar is useful for the primary isolation and differentiation of medically important Candida species.
Authors and Affiliations
Rajeshwari Prabhakar Rao
Keywords
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- EP ID EP615550
- DOI 10.18410/jebmh/2019/182
- Views 92
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