ISOLATION OF OIL DEGRADING BACTERIA FROM OIL CONTAMINATED SOIL AND EXPRESSION OF OIL DEGRADING GENES IN NON OIL DEGRADING BACTERIA
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2013, Vol 1, Issue 11
Abstract
The present study is carried out by isolation and characterization of oil degrading microbes from oil contaminated sites. In this work the Bacillus megaterium , Pseudomonas fluorescenes and S.aureus were isolated according to Bergey’s manual and further used for oil degradation to check the production of biosurfactant. The Shake flask method, Agar well diffusion method and Spread plate method were used for oil degradation. Further the optimization was carried out for best carbon, nitrogen sources and also for pH and temperature. And in last the plasmid was isolated from production media and after restriction digestion , ligation the transformation was done to transform oil degrading genes in to non oil degrading microbes.
Authors and Affiliations
Amit Pandey*| MRD LIfeSciences Pvt. Ltd, Lucknow (U.P.), 226010., Rashmi Chandra| MRD LIfeSciences Pvt. Ltd, Lucknow (U.P.), 226010.
Keywords
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