ISOLATION OF PHYTOSTEROL FROM PALM FATTY ACID DISTILLATE (PFAD) USING SOXHLET MODIFICATION
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
Objective: To isolate phytosterol compounds from PFAD to obtain pure isolates. Methods: Saponification was used to separate the sterilized part (polar extract) containing ALB and TAG with unsabbed portions (non-polar extract) containing phytosterol. This non-polar saponification extract was isolated using modified soxhlet with silica gel binder. The identification of isolate purity was conducted using Fourier Transform Infrared (FT-IR) and Gas Chromatography Mass Spectroscopy (GC-MS). Results: Saponification results obtained non-polar crude extract (unsabbed portion) with a concentration of 2,683 ppm. The result identification of phytosterol isolate with FT-IR showed the similarity of functional group between phytosterol standard and isolate. However, peak appeared in the isolate at the wave number 1741 cm-1 showing the carbonyl group C=O. Meanwhile, identification using GC-MS showed isolate purity rate was 80% to the standard. The phytosterol content contained in the isolate was 4,035 ppm. Conclusion: The results of this study indicated that the obtained phytosterol isolate had a fairly good purity with a rate of 80%. The method used is also very beneficial because it can save the solvent, increase the amount of results obtained and short in time.
Authors and Affiliations
Ovalina Sylvia Br. Ginting
Keywords
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