ISOLATION OF PHYTOSTEROL FROM PALM FATTY ACID DISTILLATE (PFAD) USING SOXHLET MODIFICATION
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
Objective: To isolate phytosterol compounds from PFAD to obtain pure isolates. Methods: Saponification was used to separate the sterilized part (polar extract) containing ALB and TAG with unsabbed portions (non-polar extract) containing phytosterol. This non-polar saponification extract was isolated using modified soxhlet with silica gel binder. The identification of isolate purity was conducted using Fourier Transform Infrared (FT-IR) and Gas Chromatography Mass Spectroscopy (GC-MS). Results: Saponification results obtained non-polar crude extract (unsabbed portion) with a concentration of 2,683 ppm. The result identification of phytosterol isolate with FT-IR showed the similarity of functional group between phytosterol standard and isolate. However, peak appeared in the isolate at the wave number 1741 cm-1 showing the carbonyl group C=O. Meanwhile, identification using GC-MS showed isolate purity rate was 80% to the standard. The phytosterol content contained in the isolate was 4,035 ppm. Conclusion: The results of this study indicated that the obtained phytosterol isolate had a fairly good purity with a rate of 80%. The method used is also very beneficial because it can save the solvent, increase the amount of results obtained and short in time.
Authors and Affiliations
Ovalina Sylvia Br. Ginting
Keywords
Related Articles
- EP ID EP635712
- DOI -
- Views 151
- Downloads 0
CONSCIOUS SEDATION WITH MIDAZOLAM AND PROPOFOL CONTROLLED BY AUDITORY EVOKED POTENTIALS INDEX DURING SPINAL ANESTHESIA
The purpose was to arrive adequate doses of midazolam and propofol infusion for sedation during spinal anesthesia, and whether AAI could be an indicator of adequate sedation level. Thirty patients aged 30 to 70 years sch...
PARAQUAT POISONING AND PARAQUAT MORTALITY IN PATIENTS REFERRED TO AHVAZ TEACHING HOSPITALS
Introduction: Today poisoning with pesticides has increased and Poisoning with paraquat is one of the most significant poisoning in patients. The aim of this study was to investigate paraquat poisoning and mortality rate...
EFFECT OF AN ANTI-ARTHRITIC HERBAL MEDICINE ON INFLAMMATORY MARKERS IN ALBINO WISTAR RATS
The anti-arthritic activity of a herbal drug was evaluated in this study. Thirty-five (35) albino wistar rats were used in this study. They were divided into seven groups, A, B, C, D, E, with seven rats each group. Group...
FORMULATION AND EVALUATION OF CANDESARTAN CO-PRECIPITATE WITH HYDROPHILIC POLYMERS; PREPARATION OF ORODISPERSIBLE TABLETS
Candesartan cilexetil is an angiotensin-receptor blocker that suffer from inadequit and variable oral bioavailability due to its poor aqueous solubility and presystemic metabolism. Therefore, the objectives of this study...
EVALUATION OF C3 COMPLEMENT AND INFLAMMATORY MARKER (C-REACTIVE PROTEIN) IN TYPE 2 DIABETES SUBJECTS WITH INSULIN RESISTANCE
Type 2 Diabetes can be caused by Insulin Resistance, a condition in which cells fail to respond to insulin properly and can progressively leads to lack of insulin. Insulin Resistance is directly interlinked with various...