Isolation of Rare Salmonella Serovars, Wangata and Penarth from Chicken in Nsukka, Nigeria
Journal Title: Microbiology Research Journal International - Year 2016, Vol 16, Issue 1
Abstract
Introduction: Salmonella infections remain a veterinary and public health problem of major importance. Rare Salmonella serovars, whose epidemiological and serological patterns are not well understood, are becoming increasingly common in Nigeria and other parts of the world. We report the isolation of Salmonella enterica serovars Wangata and Penarth, two serovars that had not been previously reported in chicken in Nigeria. Materials and Methods: A total of 300 chickens comprising of 150 intensively reared and 150 free range chickens, from selected farms and live bird markets, were sampled by cloacal swab using International Office of Epizootics (OIE) standards. Following standard bacteriological techniques, samples were pre-enriched in buffered peptone water, before transferring into Rappaport Vassiliadis medium and finally streaked onto Salmonella-Shigella agar (SSA). Salmonella spp. were identified biochemically and serotyped based on reaction with somatic (O), flagella (H), and capsular (Vi) antisera. Antibiotic susceptibility testing was performed following Kirby-Bauer (disk-diffusion) method. Results: Out of the 300 samples, 4% (n = 12) were positive for salmonellae. The isolates comprise of 6 isolates of S. enterica ser Wangata, 5 S. enterica ser Enteritidis and 1 S. enterica ser Penarth. All the rare serotypes S. Wangata and S. Penarth were isolated from free range chickens, while S. Enteritidis was isolated from both intensively reared and free range chickens. There was no difference in the sensitivity pattern between the rare serovars and serovar Enteritidis to the antibiotics tested. S. Penarth had a higher MIC to Cotrimoxazole, but lower MBC for gentamicin and tetracycline. Conclusions: Free range chickens could be vehicles for the transmission and/or reservoirs of the rare salmonellae serotypes in Nigeria. Any prophylactic program aimed at controlling these agents in poultry farms in Nigeria, must take into account the free range local chickens.
Authors and Affiliations
Okechukwu J. Obi, Anthony C. Ike, Chinasa V. Olovo
Keywords
Related Articles
- EP ID EP351960
- DOI 10.9734/BMRJ/2016/27849
- Views 131
- Downloads 0
Characterization of Psychrotrophic Molds from Indigenous Fermented Dairy Products
Aim: The aim of the study was to establish the number and type of psychrotrophic molds in Indigenous Fermented Dairy Products (IFDP) of Bengaluru market, Karnataka, India. Methodology: The study was taken up in the Depar...
Acquisition of Antibiotic Resistance in Escherichia coli Exposed To a Locally Produced Herbal Drug
Background: Antimicrobial resistance poses great threats to the treatment and eradication of pathogenic microbes. An increasing number of infectious diseases are now becoming difficult to treat in the developing world, b...
Vancomycin Resistant Enterococci Infections in Trinidad and Tobago
Aims: To document Vancomycin Resistant Enterococci (VRE) infections prevalence, risk factors, antimicrobial susceptibility patterns and evaluation of chromogenic plates in identifying VRE isolates in Trinidad and Tobago....
Relationship between Gingivitis and Autism in Children: A Matched Case-control Study
Autism is a neurodevelopmental disorder diagnosed in early childhood. Studies have shown that autistic children have poorer gingival health and hence chances of higher incidence of gingivitis may occur in autistic than n...
Diversity of Acidophilic Bacteria and Archaea and their Roles in Bioremediation of Acid Mine Drainage
Aims: To show the potential of acidophilic bacteria and archaea in bioremediation of acid mine drainage. The Mining industry generates wealth, but its long term adverse effects, which include acid mine drainage (AMD), ca...