Isolation, Partial Purification and Characterization of a Cold Active Lipase from Pseudomonas s p., Isolated from Satopanth Gla cier of Western Himalaya, India
Journal Title: International Journal of Scientific Research and Management - Year 2017, Vol 5, Issue 7
Abstract
A Psychrotrophic bacterium producing cold active lipase enzyme at low temperature was isolated from soil sample of Satopanth glacier. It wa s identified as Pseudomonas fluo rescens based on colony morphology, biochemical characteristics. Out of the seven sel ected lipase producing isolates the best one SGPR - 4 was showing activity maximum at 20 o C t emperature . The cold active lipase was purified to 6.44 fold by DEAE cellulose column chromatography. The bacterial strain SGPR - 4 showed maximum activity at pH 8.0 and temperature 20°C and it showed thermostability up to 30 o C. Therefore these properties of enzyme could be explored for industrial application and commercialization
Authors and Affiliations
Pragati Katiyar
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