Isolation, Screening and Characterization of Hydrocarbon-Utilizing Bacteria Isolated from Bitumen-Contaminated Surface Water in Agbabu, Ondo State
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 15, Issue 2
Abstract
Surface water samples from contaminated surface water in Agbabu, Odigbo Local government area of Ondo State were analyzed for hydrocarbon-utilizing bacteria. The mean total bacterial count and mean total hydrocarbon- degrading bacterial counts were determined using pour plate technique. The hydrocarbon-utilizing potentials of the isolates were further determined by screening them in minimal salt broth supplemented with 2% crude-oil over a period of 14 days. The growth of the isolates was monitored by measuring the absorbance (OD600 nm) and Total viable count (log10 CFU/ml). The effect of temperature, pH and crude-oil concentration on their degradative potentials was determined. The isolates were identified based on their cultural, morphological and biochemical characteristics according to Bergey’s Manual of Systematic Bacteriology. The Mean Total bacteria count was 7.70 (log10 CFU/ml) ranging from 6.25 to 8.77 (log 10 CFU/ml) while the Mean Total hydrocarbon-utilizing bacteria was 7.14 (log10 CFU/ml) ranging from 4.77 to 7.64 (log10 CFU/ml). Six bacteria genera isolated from the samples collected include: Staphylococcus, Micrococcus, Pseudomonas, Enterobacter, Bacillus and Alkaligens. Five isolates namely; Pseudomonas aeruginosa, Bacillus cereus, Bacillus lichenformis , Micrococcus luteus and Alkaligens faecalis showed maximum utilization of crude-oil as sole carbon source and were selected for further studies. The result of varying crude-oil concentrations (1.0 -2.5%) on each of the selected hydrocarbon-utilizing bacteria showed that the isolates grew optimally at 2% crude-oil concentrations where two of the isolates B. cereus and P. aeruginosa had the highest growth rate of 0.77 and 0.75 respectively. However, their growth gradually reduced at increased crude-oil concentration (2.5%).The growths of the isolates were optimal at neutral to alkaline pH (7-9) while their growths were greatly reduced at acidic pH 3. The optimum temperature for the growth of the isolates ranged between 40°C-45°C.The result from this research affirms that an effective utilization of crude-oil would require simultaneous action of several metabolically versatile microorganisms under favorable environmental conditions such as pH, temperature and availability of nutrient.
Authors and Affiliations
Temitayo O. Olowomofe, J. O. Oluyege, Dolapo O. Sowole
Keywords
Related Articles
- EP ID EP318149
- DOI 10.9734/JABB/2017/35414
- Views 86
- Downloads 0
Pre-treatment of Abelmoschus esculentus Seeds with Salicylic Acid and Indole Acetic Acid Enhances Nutritional Values of the Fruits
Aims: This study investigated the effects of phytohormones using indole acetic acid (IAA) and salicylic acid (SA) on some biochemical parameters on LD 88 and 47-4 genotypes of okra. Study Design: The study was carried ou...
Evolution of Lipid Oxidation in Syrian Olive Oil under Irradiation and Storage
Aims: The current study examines variations in Syrian olive oil (SOO) after gamma irradiation treatment and during storage. Study Design: Four irradiation treatments (0, 1, 2 and 3 kGy) were distributed in a completely...
Antimicrobial and Anti-inflammatory Activities of Some Marine Brown and Red Algal Species Collected from Safaga Seashore, Red Sea, Egypt
Aims: Estimation of the biological activities of 3 algal crude extracts for anti-inflammatory and antimicrobial activities. Methodology: Three marine algae were collected from Safaga seashores, the Red Sea in Egypt, extr...
Molecular Analysis and Study of Genetic Relationships among Species of Desmodium Desv. Using RAPD Markers
Aims: The present study was aimed to determine the genetic relatedness and diversity among accessions of Desmodium spp using RAPD markers. Study Design: In order to achieve the aim authentic samples of Desmodium dichotom...
Genetic Structure and Geographical Relationship of Selected Colocasia esculenta [L. Schott] Germplasm Using SSRs
Aims: SSR markers were used to infer population genetic structure variability in taro cultivars with the objective of characterizing the allelic diversity of each geographical population. Place and Duration of Study: Mas...