Isolation, screening of Aspergillus flavus and its production parameters for α- amylase under solid state fermentation
Journal Title: Journal of Applied and Natural Science - Year 2011, Vol 3, Issue 2
Abstract
The amylase producing fungi were isolated from spoiled fruits, vegetables and soil, in and around Bangalore, Karnataka, India. The isolates were identified and five fungal species were screened. The best amylase producer among them, Aspergillus sp was selected for enzyme production by both sub merged fermentation using mineral salt medium (MSM) and solid state fermentations using wheat bran as a solid substrate. The various parameters influencing solid state fermentation were optimized. The most important factors are such as pH, incubation temperature, incubation period, carbon sources, nitrogen sources and moisture content. The maximum amount of enzyme production was obtained when solid state fermentation was carried out with soluble starch as carbon source and beef extract (1% each) as nitrogen source, optimum conditions of pH 7.0, an incubation temperature of 25 (±2) °C, incubation time 96 h and 62% moisture content.
Authors and Affiliations
K. Geetha, Y. Nagaraja
Keywords
Related Articles
- EP ID EP147019
- DOI 10.31018/jans.v3i2.194
- Views 79
- Downloads 0
Musculoskeletal disorders amongst workers of glass manufacturing unit of Western Uttar Pradesh
Glass manufacturing process is a very tiresome job and the working near by the furnace makes tasks more difficult for workers as the environment is very hot. Apart from working in a hot environment various awkward postur...
Quantitative vegetation analysis of tree species in the forest adjacent to villages on the periphery of Kedarnath wildlife sanctuary
The study was carried out in the forest adjacent to three villages located on the periphery of Kedarnath Wildlife Sanctuary with the objective to evaluate the quantitative characters of tree species of the forest vegetat...
Relative toxicity of insecticides against cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera:Pseudococcidae) and its fortuous parasitod Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae)
Nineteen insecticidal formulations from 10 groups of insecticides were evaluated for their relative toxicity against cotton mealybug Phenacoccus solenopsis Tinsley and its fortuous parasitoid Aenasius bambawalei Hayat. I...
Influence of arbuscular mycorrhizal fungi and Trichoderma viride on growth performance of Salvia officinalis Linn.
Salvia officinalis (Sage) is a popular kitchen herb, member of mint (Lamiaceae) family has been cultivated for its wide range of medicinal values. Arbuscular mycorrhizae (AM) are beneficial symbionts for plant growth and...
An application of principal component analysis for pre- harvest forecast model for rice crop based on biometrical characters
An application of principal component analysis for the development of suitable statistical models for preharvest forecast of rice yield based on biometrical characters has been dealt with in the present paper. The data o...