Klebsiella: An insight into the virulence factors and antimicrobial susceptibility pattern
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2017, Vol 3, Issue 3
Abstract
Introduction: Klebsiella pneumoniae is a frequent pathogen isolated from pneumonia, urinary tract infections, liver abscesses, wound infections, intravascular catheter infections, biliary tract infections, peritonitis and meningitis. The increasing occurrence of serious Klebsiella infections necessitates the need to identify the pathogenic mechanisms that are responsible for high virulence of these strains. Objectives: Characterization, detection of virulence factors (Hypermucoviscosity, serum resistance, biofilm formation) and determination of antimicrobial susceptibility pattern of Klebsiella isolates from various clinical samples. Materials and Method: Klebsiella isolates from various clinical samples were analysed in comparison to isolates from stool samples as controls. The isolates were identified by standard microbiological techniques and their antibiogram was determined by Kirby-Bauer disc diffusion method. Biofilm production was determined by tissue culture plate method, hypermucoviscosity by string test and serum resistance by serum bactericidal assay. Results: Amongst the clinical samples, Klebsiella pneumoniae subsp aerogenes(89%) was the predominant species followed by Klebsiella oxytoca (8%) and Klebsiella pneumoniae subsp pneumoniae(3%). The stool samples exhibited a similar pattern with Klebsiella pneumoniae subsp aerogenes (86%) being the predominant species followed by K.oxytoca(14%). The antibiotic susceptibility profile of the isolates showed sensitivity to imipenem, aminoglycosides and fluoroquinolones. Hypermucoviscosity was shown by 12%, serum resistance by 41% and biofilm formation by 36% in clinical isolates. In contrast, hypermucoviscosity, serum resistance and biofilm production was observed in 6%, 7% and 16% of the control isolates respectively. Conclusion: The rising incidence of Klebsiella infections obligates an insight into the various virulence factors and drug resistance patterns to pave a way for combating the growing menace of the virulent pathogen.
Authors and Affiliations
Arpita Neogi, Rashmi K. S. , Anjana Gopi
Keywords
Related Articles
- EP ID EP305531
- DOI 10.18231/2455-6807.2017.0022
- Views 96
- Downloads 0
Seroprevalence of Hepatitis B Surface Antigen in patients attending a tertiary care hospital Valsad, South Gujarat, India
Introduction: Hepatitis B is contagious liver disease due to infection caused by Hepatitis B virus; it is spread by blood, semen or other body fluids of infected person by inoculation. Hepatitis B virus infection is wide...
A cross sectional clinicomycological study of dermatophytosis in a tertiary care hospital, North Karnataka, India
Introduction Dermatophytosis is superficial fungal infection caused by a group of keratinophilic fungi called dermatophytes capable of invading keratinized tissue of skin hair and nail The dermatophytes which cause human...
A study on the effect of storage time on Antibacterial Activity of honey
It is an age old belief that honey improves with age. In our laboratory, we conducted a study on the antibacterial property of honey against burn wound isolates. Honey freshly obtained from forest and commercially availa...
Role of Rapid diagnostic Tests for guiding therapy in partially treated malaria patients
Background: Malaria is one of the major public health problems worldwide. Diagnosis of malaria by microscopy and/ or rapid antigen detection test is the standard practice all over world. In endemic areas, many rely on cl...
Recent scenario of Opportunistic infection in HIV-infected individuals at a tertiary care hospital in Bilaspur (C.G.)
Introduction: Opportunistic Infections are now more frequent and more severe because of immunosuppression in HIV infected person. Its spectrum varies from continent to continent. The aim of present study was to document...