LIPOPEROXIDATION AND ENZYMATIVE PROCESSES IN SORGHUM PLANTS UNDER USING OF HERBICIDE AND PLANT GROWTH REGULATOR
Journal Title: Наукові доповіді НУБіП України - Year 2018, Vol 6, Issue 6
Abstract
Herbicides, as physiologically active substances, can influence the metabolism in plants, including lipoperoxidation and enzymative processes. The article presents the results of studies on the influence of biologically active substances (herbicide and plant growth regulator) on passing of lipid peroxidation oxidation and enzymative activity in Sorghum plants. The purpose of the research was to study the effects of various norms of Peak 75 WG herbicide (10; 15; 20; 25 g/ha) under different ways of using of Regoplant plant growth regulator (treatment of sowing material (250 ml/ton) and crops (50 ml/ha)) on the course of lipoperoxidation and enzymative processes in Sorghum plants. The objects of the study were Sorghum (Sorghum orysoidum) of Tytan variety, Peak 75 W.G. herbicide and Regaplant plant growth regulator. Sorghum plants were grown in accordance with the requirements of the vegetative method. Analysis in the experiments were performed on the third and fifth day after spraying the plants with studied preparations. The results of studies showed that herbicide application, both individually and in combination with plant growth regulator, significantly influenced the reaction of lipoperoxidation in Sorghum plants. The lowest level of lipoperoxidation was observed in the experiments with complex use of herbicide and plant growth regulator (treatment of seed material and application on crops). In particular, the content of malondialdehyde was lower by 15-20% in these experimental variants than in herbicide-based variants. The activity of glutathione-s-transferase in Sorghum leaves was highest in the variants of the experiment with combined application of Peak 75 WG Herbicide (10-25 g/ha) and Regoplant plant growth regulator (50 ml/ha) under pre-sowing treatment of seeds by Regroplant (250 ml/t) It was found that reducing in intensity of passing of lipoperoxidation processes under simultaneous increasing of enzymative activity was reached under introducing a mixture of Peak 75 WG herbicide (15-20 g/ha) in combination with Regoplant plant growth regulator (50 ml/ha) while pre-sowing treatment of seeds by Regoplant (250 ml/t).
Authors and Affiliations
V. P. Karpenko, S. S. Shutko
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