Loss of heterozygosity and microsatellite instability in the 3p24.2~3pter region in papillary thyroid carcinoma

Journal Title: Archives of Medical Science - Year 2007, Vol 3, Issue 3

Abstract

Introduction: The aim of this study was to analyse the loss of heterozygosity (LOH) and microsatellite instability (MSI) at the thyroid hormone receptor beta (THRB) locus (3p24.3) and in the 3p24.2~3pter region in human papillary thyroid carcinoma (PTC). Material and methods: Polymorphic cleaved amplified polymorphic sequence (CAPS) markers were used for cleaved amplified polymorphic sequence analysis to examine the THRB locus and microsatellite markers D3S1435, THRB, D3S1597 and D3S1304 were used to perform the innovative, PCR-based WAVE® Nucleic Acid Fragment Analysis to evaluate LOH/MSI presence in the 3p24.2~3pter region. Results: Twenty-three (23) PTC samples were used. Interestingly enough, only one [1] among 18 informative cases (1/18) presented with LOH at the THRB locus, while none of the same 18 cases revealed any trace of MSI. Moreover, we also observed a single polymorphism at the THRB gene, restricted to one of the used CAPS markers. Conclusions: Our results suggest that LOH and MSI in the 3p24.2~3pter region are not characteristic features in sporadic PTC.

Authors and Affiliations

Ewa Brzeziańska, Monika Migdalska-Sęk, Anna Cyniak-Magierska, Włodzimierz Koptas, Andrzej Lewiński

Keywords

Related Articles

REVIEW PAPER Trigemino-cardiac reflex: A recently discovered “oxygen-conserving” response? The potential therapeutic role of a physiological reflex

The trigemino-cardiac reflex (TCR) is defined as a sudden onset of parasympathetic dysrhythmia, sympathetic hypotension, apnea or gastric hypermotility during the stimulation of any of the sensory branches of the trige...

Postconditioning: a brief review

Preconditioning represents the most effective form of cardioprotection that can be induced to attenuate the injury accompanying a longer lasting ischemia (=index ischemia) of sufficient duration and severity to cause myo...

Unsuspected Klinefelter syndrome mosaicism presenting as osteoporosis: a case report

Hypogonadism secondary to genetic disorders like Klinefelter syndrome has been described as a cause of male osteoporosis, but the diagnosis is frequently missed or delayed because the clinical features are often subtle o...

Download PDF file
  • EP ID EP128915
  • DOI -
  • Views 161
  • Downloads 0

How To Cite

Ewa Brzeziańska, Monika Migdalska-Sęk, Anna Cyniak-Magierska, Włodzimierz Koptas, Andrzej Lewiński (2007). Loss of heterozygosity and microsatellite instability in the 3p24.2~3pter region in papillary thyroid carcinoma. Archives of Medical Science, 3(3), 192-199. https://europub.co.uk./articles/-A-128915