Measurement of Inhibition Zones, Solvent/Growth Dynamics of Adenanpthera pavonina. Linn. Against Selected Enteric Microorganisms
Journal Title: Open Access Journal of Pharmaceutical Research - Year 2024, Vol 8, Issue 1
Abstract
The aim of this study was to evaluate the inhibition zones, solvent, and growth dynamics of Seed extract of Adenanpthera Pavonina. Linn. Against selected Enteric Microorganisms. Adenanpthera Pavonina.Linn is a Red Sandalwood, (Fabaceae), an unarmed deciduous tree and its seed is traditionally used for treatment of various disease conditions. Five different solvents were used in this study: Ethyl acetate, water, absolute ethanol, fermented palm wine and distilled ethanol from palm wine). In this study, ethanol was distilled from palm wine using fractional distillation method. Palm wine solvent was fermented using barkers’ yeast (Saccharomyces cerevisiae). Isolates used in this study were obtained from Adekunle Ajasin University AkungbaAkoko Health Center (AAUHC) and were re-identified through series of biochemical test and gram staining characteristics. Isolates were identified as Enterobacter aerogenes, Burkhoderia cepacia, Acinetobacter baumanni, Pasteurella multocida, Escherichia coli, Aeromonas hydrophila, Moraxella sp., Burkhoderia pseudomallei, Enterobacter agglomerama. Extensive and series of biochemical tests were carried out to further confirm all the test bacterial strains. Biochemical tests done includes Indole test, Catalase, Citrate, Methyl Red-Voges Proskaeur (MR-VP), Triple Sugar Iron (TSI), Urease, Motility Test, Oxidase Test, to identify and characterized the test isolates, antimicrobial activity of different solvent extracts of Adenanthera pavonina Linn. Seed was tested against enteric bacterial strains by observing the zone of inhibition using agar well diffusion method. The growth dynamic of the test isolate against plant seed extract of Adenanthera pavonina Linn., was done using Ultraviolentvisible spectrophotometer. Ethanolic phytochemical analysis was done qualitatively and quantitatively. In this study, Ethyl acetate and Distilled ethanol from palm wine seed extracts of Adenanthera pavonina Linn., showed the highest antimicrobial activity against all the tested bacteria isolates. Result obtained indicates that the ethanolic, ethyl acetate and distilled ethanol seed extract of Adenanthera pavonina Linn. has antibacterial activities and can be used as antibacterial agent. It was observed that at less than an hour, Pasteurella multocida has the highest growth rate of 0.150λ and Aeromonas hydrophila have the lowest growth rate of 0.102λ. At 24 hours, Pasteurella multocida has the lowest death rate of 0.100λ and Burkhoderia pseudomallei have the highest death rate of 0.068λ Adenanthera pavonina Linn., showed the presence of secondary metabolite and its killing/growth kinetics was very outstanding which was demonstrated during this research work, the plant has a limitless antimicrobial potential, and it can be used for the development of novel antimicrobial agents.
Authors and Affiliations
Osuntokun OT, Ifedotun IT and Adeyinka A*
Keywords
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- EP ID EP751877
- DOI 10.23880/oajpr-16000307
- Views 13
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