Measurement of Serotonin Distribution and 5- Hydroxyindoleacetic Acid Excretion after Oral Administration of Serotonin using HPLC Fluorescence Detection
Journal Title: Food Science & Nutrition Technology - Year 2016, Vol 1, Issue 1
Abstract
Serotonin (5-hydroxytryptamine) is a well - known neurotransmitter with both central and peripheral functions and is involved in diverse physiological processes.The aim of this study was to determine bioavailability and overall excretion of orally administered serotonin, as well as its metabolism to 5- hydroxyindole -3 - acetic acid. Serotonin was administered to mice by oral gavage (0,1,10, and 100mg/kg body weight). Serum as well as liver and lung tissue samples were collected at 0.5, 1, 3, 6, 12, and 24 h after administration. Urine and fecal samples were collected for the entire 24 h post- administration period to determine overall excretion. Serotonin and 5-hydoxyindole-3-acetic acid levels were measured by a high-performance liquid chromatography- fluorescence detection method. The serum concentration of serotonin increased a dose-and time-dependent manner. Accumulation of serotonin was observed in the various tissues analyzed, primarily in the high dose group. Urinary 5-hydroxyindole-3-acetic acid excretion was higher in treated mice than in untreated mice; however, urinary serotonin levels were unchanged among the treatment groups. In contrast, high levels of serotonin were observed in the feces.The results of the present study provide important information for the use of orally administered serotonin to reduce risk factors associated with life style- related diseases, notably metabolic syndrome.
Authors and Affiliations
Islam J, Shirakawa H*, Aso H and Komai M
Keywords
Related Articles
- EP ID EP745952
- DOI -
- Views 29
- Downloads 0
Prevention of Allergic Diseases with Probiotic Lactic Acid Bacteria
Food allergy is an immune illness to dietary proteins, with symptoms extending from mild gastrointestinal symptoms to critical anaphylactic shock. Probiotic bacteria may advance endogenous barrier systems in patients wit...
Tocopherols and Fatty Acid Profile in Baru Nuts (Dipteryx Alata Vog.), Raw and Roasted: Important Sources in Nature that Can Prevent Diseases
Brazil has extensive biodiversity in their biomes, where the Cerrado, vegetation of the Brazilian interior, contributes a nutritional and medicinal potential still unexplored. The reduced risk of cardiovascular disease a...
Health Risks of Dietary Salt
Besides raising the blood pressure dietary salt is responsible for several other harmful effects. The most important are a number which, though independent of the arterial pressure, also harm the cardiovascular system. A...
Acceptance of Dishes based on Mechanically Separated Meat of Tambaqui (Colossoma macropomum) in a Public School, Brazil
In Brazil, due to economic and social factors, public policies are needed to grant access to quality nutrition in school. This provision should respect the local food culture; however, in the North, the region with the h...
Antioxidant and Hepatoprotective Activity of Lotus (Nelumbo Nucifera) Seed Extract
Background: Lotus the National flower of India is commonly called as Pink lotus belonging to the species Nelumbo nucifera. The seeds of the mature lotus are of great importance and are extensively used in traditional Chi...