Mechanisms of non-enzymatic antioxidant defense system of different organs of Catharanthus roseus for protective of cell membrane
Journal Title: Journal of Herbal Drugs - Year 2015, Vol 5, Issue 4
Abstract
Background & Aim: When the level of free radicals is increased and also when both the enzymatic systems and low molecular antioxidants are not sufficient to protect the organism, it seems necessary to get antioxidants from external sources. This study aimed to evaluate the antioxidant potential of different parts of Catharantus roseus Experimental: The antioxidant potential of ethanol extracts of roots, stems, leaves, flowers, seed pods and seeds of C. roseus plant were measured based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, inhibition of linoleic acid peroxidation by ferric thiocyanate (FTC), and malondialdehyde inhibition by thiobarbituric acid method (TBA) against the standards: Butylated hydroxytoluene (BHT), Butylated hydroxyanisole (BHA) and ascorbic acid. Results & Discussion: The phenolic content in the root (61.61 ± 2.58 µg of gallic acid per mg of dried weight extract) according to Folin–Ciocalteu method were more than any other organs. In scavenging a half of DPPH• free radicals (IC50), the extract of root, seed, and leaf (238.9 ± 2.02, 253.78 ± 1.42, 277.95 ± 2.56 µg mL-1), respectively, had the best performance. In the inhibition of linoleic acid peroxidation, the root extract had the best inhibitory power after ascorbic acid and BHT, and the leaf and seed extract had the performance similar to BHA. The root (78.68 ± 0.54%), and seed (77.44 ± 0.66%) had the best performance in the inhibition of MDA compared with other extracts. Pearson correlation coefficients between the phenolic content and antioxidant capacity of extracts were high and equal to -0.838, 0.895, and 0.740, respectively, according to DPPH, FTC and TBA methods. Industrial and practical recommendations: The results of this study can be promising in the potential of other applications of the plant organ especially in the inhibition of free radicals and lipids' peroxidation.
Authors and Affiliations
Shahin Mardani-Nejad; Ramazan Ali Khavari-Nejad; Sara Saadatmand; Farzaneh Najafi; Parviz Aberoomand Azar
Keywords
Related Articles
- EP ID EP23467
- DOI -
- Views 323
- Downloads 20
The effect of foliar application of chitosan on yield and essential oil of savory (Saturejaisophylla L.) under salt stress
Background & Aim: Satureja Isophylla L. is a medicinal herb which belongs to the family Lamiacease. Salinity affects the growth, the quality and quantity of essential oils of medicinal plants. Chitosan is also conside...
Antioxidant activities, polyphenolic composition and their correlation analysis on Hibiscus sabdarifa L. (sabdariffa) calices
Background & Aim: Extracts of Roselle calyx, possess compounds which could be considered as a great source of natural antioxidants. In this project, antioxidant activities, phenolic compounds content and their correlat...
Effect of ellagic acid on thiol levels in different brain tissue in an animal model of Parkinson's disease
Background & Aim: Parkinson's disease (PD) can be created with loss of dopaminergic substantial nigra neurons which is widely associated with oxidative stress and reduced glutathione (GSH), as the most important and abun...
Antidiabetic and hypolipidaemic activities of Solenostemon monostachyus
Background & Aim: Solenostemon monostachyus has remain invincible in Ibibio ethnomedicine for the management of numerous ailment including diabetes mellitus. Many of these ethnomedicinal claims are yet to be pharmacol...
Identifying some medicinal kinds at risk of extinction in Kallar Mountain in Chaharmahal va Bakhtiari province
Background & Aim: Chaharmahal va Bakhtiari province contains several major floristic plant associations of central Zagros Mountains. Kallar species were used for medicines nutrias in this area. However, these valuable sp...