METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CLOTRIMAZOLE, MICONAZOLE NITRATE, AND TINIDAZOLE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD IN TABLETS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 9
Abstract
Objective: The aim of the study is to develop and validate a high-performance liquid chromatographic method for the simultaneous determination of clotrimazole, miconazole nitrate, and tinidazole tablet dosage forms. Materials and Methods: A Waters C18 column (50 mm×4.6 mm, 5 μm) with mobile phase consisting of acetonitrile, methanol, and water 55:25:20 (v/v) (pH 2.5 adjusting with 0.5% orthophosphoric acid) was used. The flow rate was 1.0 ml/min, and effluents were monitored at 210 nm. Results: The retention time of miconazole nitrate, tinidazole, and clotrimazole tablets was found to be 2.9 min, 3.5 min, and 4.7 min, respectively. The method was validated according to the ICH guidelines for specificity, limit of detection, limit of quantification, precision, accuracy, linearity, ruggedness, and robustness. Conclusion: The method shows good reproducibility and recovery with % relative standard deviation <2. Hence, the proposed method was found to be simple, specific, precise, accurate, and linear. Hence, it can be applied for routine analysis of clotrimazole, miconazole nitrate, and tinidazole in pharmaceutical combined dosage forms.
Authors and Affiliations
DEEPIKA SHARMA , KOMAL GUPTA , POOJA CHAWLA Chawla
Keywords
Related Articles
- EP ID EP646629
- DOI -
- Views 119
- Downloads 0
PROSTATE VAPORIZATION BY DIODE LASER FOR PATIENTS WITH BENIGN PROSTATIC ENLARGEMENT
Objectives: The objectives of this study were to study the efficacy of diode laser prostatectomy in patients infected with benign prostatic enlargement.Methods: A total of 62 men were included in our prospective clinical...
ORAL CYCLOSPORINE AS AN ALTERNATIVE FOR INITIAL STANDARD PROPHYLAXIS IN ALLOGENEIC STEM CELL TRANSPLANTATION WHEN INTRAVENOUS FORMULATION IS UNAVAILABLE
Objective: The objective of the study was to compare the incidence of acute graft versus host disease (aGVHD) between oral and intravenous (IV) initial standard cyclosporine A (CsA) prophylaxis in a tertiary care center...
Enhancement of oral bioavailability via solid lipid nanoparticles of anticancer drug dasatinib - An in vitro cytotoxicity and pharmacokinetic study
Objective: Dasatinib (DST) is a BCS Class II drug having very low solubility and high permeability. Low aqueous solubility and poor dissolution of DST leads to poor bioavailability, Thus, limited aqueous solubility is th...
FABRICATION, CHARACTERIZATION, AND IN VITRO EVALUATION OF PEGYLATED GLYCERIDE LABRASOL® NANOSTRUCTURED LIPID CARRIER COMPOSITES OF METHOTREXATE: THE PATHWAY TO EFFECTIVE CANCER THERAPY
Objective: The objective of the current study is to optimize and evaluate the potential of polyethylene glycolylated (PEG) glyceride Labrasol® nanostructured lipid carrier (NLC) composites of methotrexate (MTX) to achiev...
ANALYSIS REACTIVITY OF PUNICA GRANATUM POLYPHENOLS TO THE OSTEOCALCIN, BONE MORPHOGENETIC PROTEIN-2, AND COLLAGEN TYPE-1
Objective: Punica granatum (PG) contains anthocyanins which are useful as antioxidants, anti-inflammatory, and prevent cancer, while also increasing bone cell proliferation and osteoblasts differentiation in bone remodel...