METHOD DEVELOPMENT AND VALIDATION FOR THE ASSAY OF GEMCITABINE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS BY RP-HPLC
Journal Title: Indo American Journal of Pharmaceutical Research - Year 2011, Vol 1, Issue 5
Abstract
A simple and precise, rapid and accurate RP-HPLC method has been developed and validated for the determination of gemcitabine hydrochloride in pharmaceutical dosage forms. The chromatographic separation was achieved on kromasil stainless column (150 X 4.6 mm, 5.0 μ particle size) using acetonitrile : water (40 : 60), flow rate 1.0 mL/min. The analyte was monitored using UV-Visible detector at 270 nm. The retention time of the drug was 4.093 min for gemcitabine hydrochloride. The proposed method was found to have linearity in the concentration range 80-120 μg/mL with correlation coefficient of r2 = 0.999. The method was validated for linearity, precision, LOD, LOQ and robustness. The proposed method was optimized and validated as per the ICH guidelines.
Authors and Affiliations
R. MURALI KRISHNA, M. RAMESH, T. SIVA KUMAR
Keywords
Related Articles
- EP ID EP221902
- DOI -
- Views 88
- Downloads 0
BIODEGRADABLE STARCH FOAMS AS A DRUG CARRIER
Abstract The application of aerogels as drug delivery system was successfully demonstrated for the starch. Biodegradable materials are beneficial as drug carrier as they possess the property of biodegradability. The biod...
FORMULATION AND EVALUATION OF HERBAL BASED ANTI MICROBIAL CREAM USING COW GHEE AS BASE
Abstract Essential oils like eucalyptus and neem are well known for its anti microbial activity. The aim of this study is to evaluate the anti-bacterial activity of two essential oils (eucalyptus oil,neem oil)by incorpor...
Studies on phytochemical analysis and antimicrobial activity of Artocarpus communis fruit latex against selected pathogenic microorganisms
The latex is widely used in cosmetics, pharmaceuticals and food industry as in paper, textile and petroleum industries also. The present study was carried out to assess the potential antimicrobial activity of methanolic,...
PREVALENCE OF CO-MEDICATION AND POTENTIAL DRUG-DRUG INTERACTIONS IN HUMAN IMMUNODEFICIENCY VIRUS INFECTED PATIENTS ON HIGHLY ACTIVE ANTI-RETROVIRAL THERAPY
Abstract Background:-Drug interaction is an important therapeutic challenge among Human Immunodeficiency Virus infected patients. They are often observed in these patients because they frequently receive multiple medicat...
PHARMACOLOGICAL PROPERTIES OF FERONIA LIMONIA FRUIT PULP – A REVIEW
Abstract Current research on drug development has emphasized on traditional uses of herbal constituents which were practiced in ancient times. Many parts of Feronia limonia were prescribed for diseases in ancient medicin...