METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF ACAMPROSATE CALCIUM IN TABLETS USING RP-HPLC
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: To develop an accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Acamprosate calcium (333 mg) in tablets.Methods: The optimized method uses a reverse phase column, Enable Make C18G (250 X 4.6 mm; 5μ), a mobile phase of triethylammonium phosphate buffer (pH 3.0): acetonitrile in the proportion of 30:70 v/v, flow rate of 1.0 ml/min and a detection wavelength of 210 nm using a UV detector.Results: The developed method resulted in Acamprosate calcium eluting at 2.36 min. Acamprosate calcium exhibited linearity in the range 75-225μg/ml. The precision is exemplified by relative standard deviation of 0.149%. Percentage Mean recovery was found to be in the range of 98â€102, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) was found to be 570 ng/ml and 1729 ng/ml respectively.Conclusion: An accurate, precise and linear RP-HPLC method was developed and validated for the quantitative estimation of Acamprosate calcium 333 mg in tablets as per ICH guidelines and hence it can be used for routine analysis in various pharmaceutical industries.Â
Authors and Affiliations
Jeevitha Nimma, Rajesh Tuniki, Sowjanya Kumari Andela, Sumedh Gadpale, Ashok Kumar Akuthota
Keywords
Related Articles
- EP ID EP579404
- DOI -
- Views 86
- Downloads 0
STUDY ON THE SYNTHESIS OF PUERARIN DERIVATIVES AND THEIR ANTI-VASCULAR DEMENTIA ACTIVITY
In this study, puerarin derivatives were designed by adding an active acetonitrile group that inhibits cyclooxygenase-2 (COX-2) in order to enhance the anti-vascular dementia drug activity. The acetonitrile group was lin...
ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITES FROM PITHECELLOBIUM DULCE BENTH FRUIT PEEL
Objective: The present study describes the isolation and characterization of valuable secondary metabolites from the fruit peel of Pithecellobium dulce. Methods: Solvent extraction of the plant material using methanol fo...
COMPREHENSIVE MEDICATION MANAGEMENT SERVICES IN A BRAZILIAN SPECIALTY PHARMACY: A QUALITATIVE ASSESSMENT
Objective: To understand and describe the implementation process of a comprehensive medication management (CMM) service in a public specialty pharmacy in Brazil.Methods: Ethnographic study conducted over 17 mo (September...
COMPARATIVE STUDY OF THE ANTINOCICEPTIVE ACTION OF AMITRIPTYLINE WITH FLUOXETINE AND EVALUATION OF THEIR PROBABLE MECHANISM OF THIS ACTION IN ALBINO MICE
Objective: The aim of our study was to compare the anti-nociceptive action of amitriptyline with fluoxetine and evaluation of their probable mechanism of anti-nociceptive action by observing their individual interactions...
CLOVE OR GREEN TEA ADMINISTRATION ANTAGONIZES KHAT HEPATOTOXICITY IN RATS
Objective: Khat consumption has become a common problem that affects the health aspects of life in Yemen and other parts in the world. The liver has been suspected to be particularly vulnerable to the harmful effects of...