Method development and validation using HPLC technique – A review
Journal Title: Journal of Drug Discovery and Therapeutics - Year 2014, Vol 2, Issue 10
Abstract
Analytic method development and validation are key elements of any pharmaceutical development program. HPLC analysis method is developed to identify, quantity or purifying compounds of interest. This article mainly focuses on the optimization of HPLC conditions and other important perspectives during method development and validation. Various critical steps related to analytical method development and validation is discussed. A sequence of events required for method development and analytical validation are described. The steps involved in developing a stability-indicating HPLC method influences the analysis of degradation products/impurities in stability study and its validation demonstrate the suitability for its intended purpose.
Authors and Affiliations
Suman Sood| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, Rajni Bala| Department of Pharmaceutics, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India, N. S. Gill| Department of Pharmacology, Rayat Institute of Pharmacy, Rail Majra S.B.S Nagar, Punjab, India
Keywords
Related Articles
- EP ID EP1545
- DOI -
- Views 445
- Downloads 26
The Phytochemical Constituents of Withania Somnifera (Ashwagandha)
Withania somnifera is a well known Indian medicinal plant widely used in the treatment of many clinical conditions in India. Unani system of medicine, roots of Withania somnifera commonly known as Asgand are used for the...
IMPORTANCE OF GARLIC (ALLIUM SATIVUM): AN EXHAUSTIVE REVIEW
Allium sativum, also known as Clove Garlic is part of the Lilliceae plant family. Garlic is a member of the onion family and is one of nature's most versatile medicinal plant. The name “Allium sativum” is derived from th...
PHYTOCHEMICAL AND ANTIMICROBIAL INVESTIGATION OF NYMPHAEA NAUCHALI LEAF NATIONAL FLOWER OF BANGLADESH
The methanolic extracts of Nymphaea nauchali was evaluated for antimicrobial activity against clinically significant bacteria Staphylococcus aureus, Pseudomonas aureus, Pseudomonas aeruginosa, Bacillus subtilis, Bacilliu...
ARTEROLANE MALEATE AND PIPERAQUINE PHOSPHATE: A NEW OPTION IN THE TREATMENT OF PLASMODIUM FALCIPARUM MALARIA
Malaria is holoendemic in India, claiming thousands of deaths annually. Recent data suggests that the burden of malaria, the most significant parasitic disease in the world, is increasing. India’s expansive geography and...
FORMULATION DEVELOPMENT AND CHARACTERIZATION OF FAST DISSOLVING ACETAMINOPHEN TABLETS
The aim of the present work is to formulate a tablet which disintegrate and dissolve rapidly and give its rapid onset of action. Acetaminophen is an analgesic, antipyretic drug having bitter taste. In present study an at...