Microarrays – new possibilities for detecting biological factors hazardous for humans and animals, and for use in environmental protection
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2016, Vol 23, Issue 1
Abstract
Both the known biological agents that cause infectious diseases, as well as modified (ABF-Advanced Biological Factors) or new, emerging agents pose a significant diagnostic problem using previously applied methods, both classical, as well as based on molecular biology methods. The latter, such as PCR and real-time PCR, have significant limitations, both quantitative (low capacity), and qualitative (limited number of targets). The article discusses the results of studies on using the microarray method for the identification of viruses (e.g. Orthopoxvirus group, noroviruses, influenza A and B viruses, rhino- and enteroviruses responsible for the FRI (Febrile Respiratory Illness), European bunyaviruses, and SARS-causing viruses), and bacteria ([i]Mycobacterium spp., Yersinia spp., Campylobacter spp., Streptococcus pneumoniae, Salmonella typhi, Salmonella enterica, Staphylococcus aureus, Neisseria meningitidis, Clostridium difficile , Helicobacter pylori[/i]), including multiple antibiotic-resistant strains. The method allows for the serotyping and genotyping of bacteria, and is useful in the diagnosis of genetically modified agents. It allows the testing of thousands of genes in one experiment. In addition to diagnosis, it is applicable for gene expression studies, analysis of the function of genes, microorganisms virulence, and allows the detection of even single mutations. The possibility of its operational application in epidemiological surveillance, and in the detection of disease outbreak agents is demonstrated.
Authors and Affiliations
Tomasz Mirski, Michał Bartoszcze, Agata Bielawska-Drózd, Romuald Gryko, Janusz Kocik, Marcin Niemcewicz, Krzysztof Chomiczewski
Keywords
Related Articles
- EP ID EP79309
- DOI 10.5604/12321966.1196849
- Views 126
- Downloads 0
Determining the scale of designer drugs (DD) abuse and risk to public health in Poland through an epidemiological study in adolescents
Since 2008, it has been recognised by most health authorities worldwide that the abuse of newly-emerging psychoactive drugs, (‘designer drugs’/‘legal highs’; DD), in youth is a rapidly increasing problem, especially in t...
Phenological changes in olive (Ola europaea L.) reproductive cycle in southern Spain due to climate change
Introduction. Modifications of crop species phenology due to a changing environment are of interest because of their impact on fruit set and final harvest. Pre-flowering and flowering phenophases in olive groves at diffe...
The influence of passive and active moral training on medical university on changes of students’ moral competence index – results from randomized single blinded trial
Introduction and Objectives. Human’s life as well as medical professions consist of many moral dilemmas. The aim of our study was to evaluate moral competences of midwifery students during their whole university educatio...
Prevalence of tick-borne pathogens in an urban park in Rome, Italy
[b]introduction.[/b] Limited information is available about the presence of tick-borne pathogens in urban parks in Italy. To fill this gap, ticks were collected in a public park in Rome over a 1-year period and screened...
Professional communication competences of nurses – a review of current practice and educational problems
Background: A dissonance between high ‘technical’ professionalism of nurses and a relatively low level of patient satisfaction with received care is a phenomenon observed in many countries.Method: Theoretical concept and...