Microbiological analysis of common preservatives used in food items and demonstration of their in vitro anti-bacterial activity

Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 6

Abstract

Objective: To quantify the microorganisms contaminating the common preservatives used in food as well as to detect their in vitro anti-bacterial traits. Methods: A total of 9 preservatives were subjected to conventional cultural and biochemical methods for microbial enumeration. Anti-bacterial activities were demonstrated through the agar well diffusion method. Results: All samples were found to be contaminated with bacteria up to 105 CFU/g and with the fungal flora within a range of 1 01-1 02 CFU/g. Escherichia coli, Pseudomonas spp. and Staphylococcus spp. were demonstrated in most of the samples. Sodium sulfite and citric acid possessed the strongest anti-bacterial trait against all of the test bacteria. Acetic acid exhibited activity against 6 out of 8 test bacteria while vinegar exhibited the activity against 4 bacteria. Activity of salt was demonstrated only against Listeria spp. and Bacillus spp., while activity of sugar and honey was found only against Escherichia coli and Klebsiella spp., respectively. Conclusions: According to the current investigation, sodium sulfite and citric acid samples were found to be satisfactory preservatives both in terms of microbiological criteria and their antibacterial traits.

Authors and Affiliations

Keywords

Related Articles

Repellent activity of Ageratum houstonianum Mill. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae)

Objective: To determine the repellent activity of hexane, ethyl acetate and methanol crude extracts of Ageratum houstonianum (A. houstonianum) leaves against adult Anopheles stephensi (An. stephensi), Aedes aegypti (Ae....

Mosquito larvicidal and biting deterrency activity of bud of Polianthes tuberosa plants extract against Anopheles stephensi and Culex quinquefasciatus

Objective: To evaluate the larvicide and biting deterrency activity of bud of Polianthes tuberosa (P. tuberosa) against Culex quinquefasciatus (Cx. quinquefasciatus) and Anopheles stephensi (An. stephensi). Methods: Crud...

In vitro antibacterial potential of Hydrocotyle javanica Thunb.

Objective: To explore the in vitro antimicrobial potential of Hydrocotyle javanica (H. javanica) Thunb. against human pathogenic bacteria and to standardize purification protocol to harvest the active principle in large...

Ectoparasite of Tupaia glis (Scandentia: Tupaiidae) from Lingai agricultural area, Terengganu

Objective: To investigate ectoparasite fauna on the common tree shrew [Tupaia glis (T. glis)] in Lingai agriculture area, Terengganu. Methods: The sampling was conducted once a month with five consecutive days from Novem...

Antagonistic activity of marine sponge associated Streptomyces sp. against isolated fish pathogens

Objective: To investigate the antibacterial potential of the marine actinomycetes isolated from sponge samples. Methods: Thirty six marine sponge samples were collected from Palk Strait and further used for actinomycetes...

Download PDF file
  • EP ID EP229102
  • DOI 10.1016/S2222-1808(14)60605-8
  • Views 89
  • Downloads 0

How To Cite

(2014). Microbiological analysis of common preservatives used in food items and demonstration of their in vitro anti-bacterial activity. Asian Pacific Journal of Tropical Disease, 4(6), 452-456. https://europub.co.uk./articles/-A-229102