Micropropagation of Cerasus avium (L.) Moench in vitro
Journal Title: Лісівництво і агролісомеліорація - Year 2014, Vol 124, Issue
Abstract
The paper deals with peculiarities of micropropagation of cherry (Cerasus avium (L.) Moench). The potential for initiating and proliferating of shoot cultures derived from C. avium and a micropropagation method for these species was developed. The high efficiency of the method for the foundation stock sterilization for introduction into culture in vitro developed by Laboratory of Forest Tree Breeding of URIFFM earlier was identified. Shoot apices and axillary buds were collected in laboratories from greenhouse-grown one-year-old trees of cherry. Nodal segments, 0,5-1 cm in length, without leaves were then excised from the shoots. The effects of different plant growth regulators in micropropagation and medium that encourages axillary bud development were investigated. In order to identify the influence of apical dominance on the intensity of formed shoots surveys separately for the terminal and axillary buds are being conducted. Morphogenesis activity for explants of green nodal segments of shoots and apical meristem was high (60.8 to 100 %) regardless of the type of phytohormones. In proliferation stage, multiplication rate of about 1,4 was achieved over a 8 week period using MS medium with 0,3 mg l -6-Benzylaminopurine.
Authors and Affiliations
N. Vysotska
Keywords
Related Articles
- EP ID EP180814
- DOI -
- Views 137
- Downloads 0
Resin productivity of scots pine at different type of spatial distribution of trees in Small Polissya
The paper presents the results of investigation of the spatial structure of tapped Scots pine. Peculiarities of distribution of the trees of different resin productivity categories are revealed. Resin productivity depend...
The assessment of ecotopes' environment satisfactoriness and predictive modelling of conditions for coenopopulations of rare forest flora species (the case of Tulipa quercetorum Klock. Et Zoz.)
Introduction. The integrated procedure of the assessment of ecotopes’ environment satisfactoriness, adaptive potential of the rare forest flora species and predictive modelling of conditions of their coenopopulations wer...
Influence of early thinning on condition of artificial stands in reclamated lands
Influence of early thinning on condition of artificial stands in lands, reclamated after mining operations by digging is analyzed on territory of Zhitomir Polissya. Height of plantations without thinning was less, and di...
Application of mineral and organic substances for pine bark bug control
Introduction The pine bark bug Aradus cinnamomeus Panzer (Heteroptera, Aradidae) inhabits forest plantations of Scots pine (Pinus sylvestris L.) at the age of 5 years, which leads to deterioration of their health and gr...
Complex assessment of perspectives of Douglas fir (Pseudotsuga Menziesii (Mirb.) Franco) in forest-steppe conditions of Ukraine
Now, during the search of the reasonable forest-breeding approaches to increase forest plantations productivity, trials of introduced coniferous species are relevant with a view to identifying high-productive, fast-growi...