MICROWAVE-ASSISTED SYNTHESIS OF AGNP USING AQUEOUS LEAVES EXTRACT OF VINCA ROSEA AND ITS THERAPEUTIC APPLICATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 12
Abstract
Objective: Green synthesis of silver nanoparticles was attempted with the help of aqueous Vinca rosea leaf extract. The aim of the study was to combine the therapeutic activity of Vinca rosea and the deep tissue penetration capabilities of the silver nanoparticles.Methods: This study focuses on the green synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Vinca rosea leaves, its characterization, and evaluation of its antibacterial and anticancer activity by diffusion method and MTT assay using human lung carcinoma cell line A549 respectively. The nanoparticles were synthesised by exposing the reaction mixture containing silver nitrate and Vinca leaf aqueous extract to microwave radiation.Results: The characterization of synthesised nanoparticles was carried out by observing the peaks on scanning from 250 to 800 nm using UV spectroscopy, the end point for the complete formation of nanoparticles marked by a colour change to reddish brown. Dynamic Light Scattering (DLS) which evaluated particle size uniformity and Scanning Electron Microscopy (SEM) which determines the particle size revealed that the nanoparticles were spherical in shape and measured an average of 50.75 nm. 170µg/ml of AgNPs of Vinca leaf aqueous extract should potent anti-bacterial activity tested by agar well diffusion method as well as the cytotoxic activity which was evaluated by MTT assay.Conclusion: The synthesised nanoparticles were found to be potentially cytotoxic against A549 cell line and also demonstrated anti-bacterial activity. The activity may be attributed to the fact that silver ions are known to impair macromolecules containing sulphur and phosphorus like protein and DNA owing to their small size and high penetration power.Â
Authors and Affiliations
Sweta Patel, Aishaani Maheshwari, Amrish Chandra
Keywords
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