Modulating activity of M1 receptor to the reaction of ileal smooth muscle
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2011, Vol 65, Issue 0
Abstract
Background: The subject of the study was determination of the effect of drugs on ileal smooth muscle contraction induced by activation of M1 type muscarinic receptors. Drugs that have an effect on muscarinic receptors are divided to agonists, with close ties to the receptor and high internal activity and antagonists, with no internal activity. Conducted experiments tested interactions between a broad-spectrum agonist of muscarinic receptors, carbachol and a selective muscarinic receptor antagonist of M1 type, pirenzepine. Material/Methods: Testing was conducted on tissues isolated from rat’s intestine. Male Wistar rats with weight between 220 g and 360 g were anesthetized by intraperitoneal injection of urethane (120 mg/kg). Concentration-effect curves were determined with the use of cumulated concentration method, in accordance with the van Rossum method (1963) in Kenakin modification (2006). Results: The purpose of the study was determination of concentration-effect curves for carbachol. This curve was compared with the curve of receptor occupation depending on concentration of this drug. Based on concentration-effect curves, the average value of EC50 was calculated for carbachol, amounting to 2.44×10–6 [M/l]. Conclusions: The results confirmed that atropine is effective in stopping contractions caused by carbachol, meeting the conditions of competitive antagonists. Atropine caused the shift of curves for carbachol to the right. Pirenzepine, selectively blocking muscarinic receptors of M1 type gave similar results. It was proved that in the preparation of gastric fundus smooth muscle, M1 type receptors occur not only presynaptically, but also postsynaptically.
Authors and Affiliations
Izabela Glaza, Leszek Szadujkis-Szadurski, Rafał Szadujkis-Szadurski, Marta Gajdus, Joanna Olkowska
Keywords
Related Articles
- EP ID EP66510
- DOI -
- Views 165
- Downloads 0
Znaczenie zegara biologicznego w utrzymaniu homeostazy glukozy
Mechanizm działania zegara biologicznego polega na rytmicznej ekspresji genów zegarowych, regulujących aktywację wybranych składników genomu. Dzięki transkrypcyjno-translacyjnym powiązaniom dochodzi do endogennego rytmu...
Yamanaka’s factors and core transcription factors – the molecular link between embryogenesis and carcinogenesis
Oct4 and Sox2 transcription factors (belonging to the Yamanaka’s factor family) and Nanog, named together as core transcription factors of pluripotency, are indispensable to induce and maintain the pluripotency state. Th...
Czy mutacje w genie kodującym czynnik transkrypcyjny EKLF (Erythroid Krüppel-Like Factor) mogą chronić nas przed chorobami zakaźnymi i pasożytniczymi?
Czynnik transkrypcyjny EKLF (Erythroid Krüppel-Like Factor) należy do grupy czynników Krüppel-podobnych, które regulują proliferację, różnicowanie, rozwój i apoptozę komórek w organizmach ssaków. Czynnik EKLF występuje w...
The role of metalloproteinases in modification of extracellular matrix in invasive tumor growth, metastasis and angiogenesis
Extracellular matrix metalloproteinases (MMPs) are a family of endopeptydases which recquire a zinc ion at their active site, for proteolityc activity. There are six members of the MMP family: matrilysins, collagenases,...
PARP inhibitors – theoretical basis and clinical application
Poly-ADP-ribose polymerases (PARP) are involved in a number of processes that are vital for every living cell. Once activated by the presence of DNA damage they trigger poly-ADP-ribosylation of various proteins which ar...