Modulatory Effect of Ethanolic Leaf Extract of Annona muricata Pre-treatment on Liver Damage Induced by Dimethylnitrosamine (DMN) in Rats
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 8, Issue 3
Abstract
Aim: This study aim to evaluate the modulatory effect of ethanolic leaf extract of Annona muricata against dimethylnitrosamine (DMN)-induced hepatic toxicity in rats. Methodology: Twenty-four (24) male albino rats divided into four (4) groups of six (6) rats each were used for the study. Group 1 served as control and was untreated, group 2 and 3 were pre-treated with 400 mg/kg Annona muricata for one week while group 3 and 4 each received a single oral dose of 20 mg/kg DMN after one week. The rats were sacrificed 48 hrs after DMN administration. Place and Duration of Study: University of Benin and Benson Idahosa University, Benin city, between January and April, 2015. Results: In rats administered 20 mg/kg DMN, Annona muricata pre-treatment at 400 mg/kg body weight significantly reduced the levels of alanine aminotransferase (ALT), aspartate amino-transferase (AST), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), total cholesterol (TC), triglyceride (TG) and malondialdehyde (MDA) as well as significantly increased reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) compared to DMN-alone administered rats which showed the opposite. Histopathological study in corroborating biochemical analysis also showed significant reduction of liver damage in the rats. Conclusion: Annona muricata significantly restored the antioxidant levels in the liver, counteracted oxidative hepatic dysfunction and exhibited significant protective effect against DMN-induced liver toxicity, which can be mainly attributed to the antioxidant property of Annona muricata.
Authors and Affiliations
Usunobun Usunomena, Okolie P. Ngozi, Eze G. Ikechi
Keywords
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- EP ID EP344428
- DOI 10.9734/BJPR/2015/19841
- Views 103
- Downloads 0
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