MOLECULAR-BIOLOGYCAL CHARACHTERISTICS AND IDENTIFICATION OF BACILLUS SP. ONU14 STRAIN WITH ENTOMOPATHOGENIC ACTIVITY
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 1
Abstract
Aim. To study molecular-biological characteristics and Bacillus sp. ONU14 strain with entomopathogenic activity against mushrooms pest Bradisia pilistriata Frey. Methods. The identification methods were FAMES analysis by GC with MIDI Sherlock identification system and multiplex PCR with group-specific primers. The molecularbiological characteristics were studied with phase-contrast microscopy, electron microscopy, SDS-PAGE and gradient ultracentrifugation. Results. Fatty acids composition and multiplex reaction products identify the strain as Bacillus thuringiensis. Intracellular crystals have irregular shape and form protoxins with 65 and 25 kDa. Conclusions. Endospore-forming Bacillus sp. ONU14 strain with entomopathogenic activity was characterized by the microbiological and molecular-biological methods. Fatty acids composition and multiplex PCR reaction identified it as Bacillus thuringiensis.
Authors and Affiliations
A. M. Ostapchuk
Keywords
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- EP ID EP370584
- DOI 10.18524/2307-4663.2015.1(29).48011
- Views 87
- Downloads 0
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