Molecular Characterization and SCCmec in Methicillin Resistant Staphylococcus aureus from Healthy Worker Nasal Swab in Larestan Hospital
Journal Title: International Journal of Medical Laboratory - Year 2016, Vol 3, Issue 3
Abstract
Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was performed in all samples. Also, agar screen plate with oxacillin was carried out using CLSI guidelines. The two methods were then compared. Results: Of 250 samples, 37 (14.8%) samples are Staphylococcus aureus, 28 (75.7%) of samples were confirmed at Methicillin-resistant Staphylococcus aureus (MRSA) harboring mec A gene detected by Multiplex polymerase chain reaction (PCR) and 9 (24.3%) negative mec A. Sixteen of 28 (57.1%) were HA-MRSA and the remaining 12 (42.8%) were CA-MRSA. The Multiplex PCR assay for SCCmec complex of MRSA strains showed that 9 (32.1%) samples were SCCmec type I, 8 (28.6%) SCCmec type IVb, 5 (17.9%) SCCmec type II, 4 (14.3%) SCCmec type V and 2 (7.1%) SCCmec type III. Agar screen plate with oxacillin was found in all the 28 MRSA samples to harbor mecA gene and all was resistant. Conclusion: Our results illustrated that more than 70% of staph aureus strains were positive for mec A gene and more than 50% of them were HA-MRSA. In comparison to other methods, PCR and Agar screen method more sensitivity determines MRSA isolates. However, PCR was identified as the ideal method for detecting MRSA strains.
Authors and Affiliations
Mehdi Ebadi, Hossein Faramarzi
Keywords
Related Articles
- EP ID EP212555
- DOI -
- Views 108
- Downloads 0
Effect of Repeated Injection of Cadmium on Bax/Bcl-2 mRNA Level in Stomach of Rats
Background and Aims: Cadmium is an important environmental pollutant and a potent toxicant to organisms. However, the toxicity of Cadmium and its influences on stomach is still unclear. We examined the effects of intrape...
Analysis of clbN and clbB genes in Isolated Klebsiella pneumonia of Biopsies from Patients with Colorectal Cancer
Background and Aims: Recently it has been proved that some of Enterobacteriacea like Klebsiella pneumoniae, which carry PKS islands, damage dsDNA by encoding Colibactin genotoxin; and finally they induce some apoptosis i...
Anti-viral Effect and Mechanism of Carvacrol on Herpes Simplex Virus Type 1
Background and Aims: Herpes simplex virus type 1 (HSV-1) belonging to herpes viridae family is a normal human pathogen with benign lesions in immunocompromised patients which creates serious problems. Acyclovir is used t...
Physiological and Pathological Roles for MicroRNAs: Implications for Immunity Complications
MicroRNAs (miRNAs) are small non-coding regulatory RNAs molecules with a size of approximately 22 nucleotides that are implicated in regulating gene expression at the post-transcriptional regulatory levels. Inflammatory...
Evaluating the Coconut Water as a Replacement for the Fetal Calf Serum in Cultivation of Promastigotes of Leishmania Infantum
Backgrounds and Aims: The protozoan parasites of the genus Leishmania can be stated as the causative agents of the various clinical diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, whic...