Molecular Characterization and SCCmec in Methicillin Resistant Staphylococcus aureus from Healthy Worker Nasal Swab in Larestan Hospital
Journal Title: International Journal of Medical Laboratory - Year 2016, Vol 3, Issue 3
Abstract
Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital. Material and Methods: This study was carried out from the 250 nasal swab healthy worker at Larestan hospital. Multiplex polymerase chain reaction for mecA gene was performed in all samples. Also, agar screen plate with oxacillin was carried out using CLSI guidelines. The two methods were then compared. Results: Of 250 samples, 37 (14.8%) samples are Staphylococcus aureus, 28 (75.7%) of samples were confirmed at Methicillin-resistant Staphylococcus aureus (MRSA) harboring mec A gene detected by Multiplex polymerase chain reaction (PCR) and 9 (24.3%) negative mec A. Sixteen of 28 (57.1%) were HA-MRSA and the remaining 12 (42.8%) were CA-MRSA. The Multiplex PCR assay for SCCmec complex of MRSA strains showed that 9 (32.1%) samples were SCCmec type I, 8 (28.6%) SCCmec type IVb, 5 (17.9%) SCCmec type II, 4 (14.3%) SCCmec type V and 2 (7.1%) SCCmec type III. Agar screen plate with oxacillin was found in all the 28 MRSA samples to harbor mecA gene and all was resistant. Conclusion: Our results illustrated that more than 70% of staph aureus strains were positive for mec A gene and more than 50% of them were HA-MRSA. In comparison to other methods, PCR and Agar screen method more sensitivity determines MRSA isolates. However, PCR was identified as the ideal method for detecting MRSA strains.
Authors and Affiliations
Mehdi Ebadi, Hossein Faramarzi
Keywords
Related Articles
- EP ID EP212555
- DOI -
- Views 115
- Downloads 0
Effect of Lactobacillus delbrueckii on Blood Sugar in Diabetic Mice
Background and Aims: Probiotics are defined as live microorganisms that are beneficial to human health. Probiotics are commonly found in some foods such as dairy products or complementary tablets. A recent research shows...
Analysis of clbN and clbB genes in Isolated Klebsiella pneumonia of Biopsies from Patients with Colorectal Cancer
Background and Aims: Recently it has been proved that some of Enterobacteriacea like Klebsiella pneumoniae, which carry PKS islands, damage dsDNA by encoding Colibactin genotoxin; and finally they induce some apoptosis i...
Molecular Characterization and SCCmec in Methicillin Resistant Staphylococcus aureus from Healthy Worker Nasal Swab in Larestan Hospital
Background and Aims: The aim of present study was to investigate the molecular characteristic of Staphylococcus aureus to detect mec A gene and to type SCCmec in strain isolated from healthy worker at Larestan Hospital....
Comparison the Occurrence Rate of Oral Candida Species in Edentulous Denture Wearer and Dentate Subjects
Background and Aims: Different Candida species, especially Candida albicans have been known as part of human oral cavity normal flora. Changes in the oral environment resulting from tooth loss or denture application can...
Evaluation of MLL2 (KMT2B) Gene Expression in Colorectal Cancer Tissue Compared with Adjacent Tumor Free Tissue
Background and Aims: An impairment of expression in MLL2 gene involved in histone methylation induces alteration in the methylation patterns at enhancers and causes several cancers including colon cancer. In this study,...