Molecular Characterization of TEM, SHV and CTX-M Extended Spectrum Beta Lactamase Among Escherichia coli And Klebsiella pneumoniae In Urinary Isolates.
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 10
Abstract
Objective: To determine the prevalence and molecular characterization of ESBLs produced by E.coli and K.pneumoniae from urinary isolates. Material And Method: Urinary isolates of patients in Rama Medical College, Kanpur (U.P), India from November 2015 to December 2016. ESBLs were determine by disk diffusion test , double disk diffusion test, Etestand Vitek 2 ESBLs .All ESBLs producing isolates were investigated for the presence of bla(TEM), bla(SHV) and bla(CTX-M) genes by polymerase chain reaction (PCR). Results: Total of 341 isolates of Escherichia coli and Klebsiella pneumoniae isolated from urinary isolates were processed. 207 were E. coli and 134 were K. pneumoniae. ESBL screening test was performed. Of the 90 screening test positive K. pneumoniae isolates, ESBL production was detected in 48.8% (44/90) by E-test and the beta-lactamase genes were bla(SHV) (34/90, 37.7%), bla(TEM) (25/90, 27.7%), and bla(CTX-M-like) (35/90, 38.8%) and for 161 screening test positive E. coli, ESBL production was detected in 36.6% (59/161) of E. coli that carried bla(TEM) bla(CTX-M-like) and bla(SHV) genes in 5.5% (9/161), 28.57% (46/161) and 22.36% (36/161), respectively. Conclusion: The double disk diffusion test should be added routinely in the antibiotic susceptibility test for the Enterobacteriaceae. It is simple to perform, easy to interpret, and economical. The presence of bla(CTX-M), bla(SHV) and bla(TEM) in ESBL-producing E. coli and K. pneumoniae indicastes the high prevalence of these genes in North India.
Authors and Affiliations
R. Sujatha, Anil Kumar
Keywords
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